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Related Experiment Video

Updated: May 9, 2025

Differentiation and Imaging of Brown Adipocytes from the Stromal Vascular Fraction of Interscapular Adipose Tissue from Newborn Mice
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Luteolin, as a bidirectional ROS regulator, elevates mouse beige adipocyte browning.

Zhixin Zhang1, Linli Zhao1, Jiahui Wang1

  • 1School of Food and Biological Engineering, Hefei University of Technology, Hefei, Anhui 230009, China.

Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids
|May 1, 2025
PubMed
Summary
This summary is machine-generated.

Luteolin enhances beige adipocyte thermogenesis by differentially regulating reactive oxygen species (ROS) in adipocytes and mast cells. This natural compound promotes adipocyte browning via the Nrf2/Catalase pathway, counteracting mast cell-mediated inhibition.

Keywords:
AdipocyteBrowningLuteolinMast cellReactive oxygen species

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Area of Science:

  • Metabolism and Endocrinology
  • Cell Biology
  • Immunology

Background:

  • Thermogenesis in beige adipocytes relies on reactive oxygen species (ROS).
  • Mast cell activation by ROS can inhibit beige adipocyte differentiation and function.
  • Luteolin, a natural antioxidant, may modulate both adipocyte and mast cell responses.

Purpose of the Study:

  • To investigate luteolin's effect on ROS levels in beige adipocytes and mast cells.
  • To determine how luteolin influences mast cell-mediated inhibition of adipocyte browning.
  • To elucidate the molecular mechanisms underlying luteolin's actions.

Main Methods:

  • Differentiation of mouse stromal vascular fraction (SVF) cells into beige adipocytes.
  • Activation of mouse bone marrow-derived mast cells (BMMCs) with hydrogen peroxide (H2O2).
  • Assessment of intracellular ROS levels, gene expression, and cellular functions.

Main Results:

  • Luteolin increased ROS and thermogenic gene expression in beige adipocytes, unlike N-acetylcysteine (NAC).
  • Luteolin inhibited H2O2-induced mast cell activation and ROS generation.
  • Luteolin partially reversed mast cell-induced inhibition of beige adipocyte differentiation and thermogenesis.
  • Luteolin's effects were mediated by the Nrf2/Catalase pathway.

Conclusions:

  • Luteolin differentially regulates ROS in beige adipocytes and mast cells.
  • Luteolin promotes adipocyte browning by enhancing thermogenesis and inhibiting mast cell-driven suppression.
  • The Nrf2/Catalase pathway is a key mediator of luteolin's dual action.