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Updated: May 22, 2025

A Multiplexed Luciferase-based Screening Platform for Interrogating Cancer-associated Signal Transduction in Cultured Cells

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Published on: July 3, 2013

Chemically Synthesized Fluorescence-Based Kinase Sensing Systems for Signaling in Cancer.

Anindita Roy¹, Monisha Singha², Subhankar Singha¹

¹Centre for Interdisciplinary Sciences (CIS), JIS Institute of Advanced Studies and Research (JISIASR), JIS University, Howrah, West Bengal, 711112, India.

Chembiochem : a European Journal of Chemical Biology

|May 2, 2025

Summary

This summary is machine-generated.

Chemically synthesized fluorescence-based kinase sensors offer sensitive, real-time detection of kinase activity. These versatile tools aid in understanding cancer biology and developing personalized medicine by identifying biomarkers and optimizing therapies.

Keywords:

cancer diagnostics fluorescence sensing kinase detections kinase inhibitors kinase substrates

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Mimicking the Function of Signaling Proteins: Toward Artificial Signal Transduction Therapy

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Published on: September 29, 2016

Area of Science:

Biochemistry
Molecular Biology
Chemical Biology

Background:

Kinases regulate crucial cellular processes via phosphorylation.
Dysregulated kinase activity is linked to cancer development, progression, and treatment resistance.
Accurate monitoring of kinase activity is vital for cancer research and clinical applications.

Purpose of the Study:

To review chemically synthesized fluorescence-based kinase sensing systems.
To highlight their advantages over genetically encoded systems for cancer research.
To explore their potential in diagnostics and personalized cancer therapy.

Main Methods:

Focus on chemically synthesized fluorescence-based kinase sensing systems.
Classification into peptide substrate-based, ATP/ADP-recognition-based, and inhibitor-based systems.
Discussion of design, mechanisms, and applications in cancer biology.

Main Results:

Chemically synthesized sensors offer precise control and versatility for in vitro and in vivo studies.
These systems provide sensitive, real-time insights into kinase function.
They are well-suited for quantitative, high-throughput screening.

Conclusions:

Chemically synthesized fluorescence-based kinase sensors are powerful tools for cancer research.
They enable identification of kinase biomarkers and optimization of targeted therapies.
These sensors advance personalized medicine by providing detailed kinase activity profiles.