Related Experiment Video
Updated: May 14, 2025

Detection of Small GTPase Prenylation and GTP Binding Using Membrane Fractionation and GTPase-linked Immunosorbent Assay
Published on: November 11, 2018
PLEKHG2 and the PLEKHG family: Linking Rho family GTPases to neural development and disorders
Katsuya Sato1, Masashi Nishikawa2, Koh-ichi Nagata3,4
1Department of Molecular Pathobiochemistry, Gifu University Graduate School of Medicine, Yanagido, Gifu, Japan.
Abstract:
Rho family small GTPases (Rho GTPases) are key regulators of cellular morphology, primarily through their control of the actin cytoskeleton. They play crucial roles in various cellular processes, including cell division, adhesion, and migration. The activity of Rho GTPases is tightly regulated by specific guanine nucleotide exchange factors (GEFs), which facilitate the exchange of GDP for GTP, thereby activating the GTPases. In the human genome, RhoGEFs are categorized into two major families: the DOCK family, comprising 11 members characterized by dedicator of cytokinesis (DOCK) homology regions, and the Dbl family, consisting of 64 members that contain a diffuse B-cell lymphoma (Dbl) homology domain. This review focuses on the pleckstrin homology and RhoGEF domain containing G (PLEKHG) family within the Dbl family of RhoGEFs, which remains largely uncharacterized. We summarize their structure and function, with a particular emphasis on PLEKHG2, discussing its regulatory mechanisms, interactions with various molecules, and its involvement in neural functions.
More Related Videos
Related Concept Videos
Small GTPases - Ras and Rho
Three regulatory proteins control their activity:
Cell Polarization by Rho Proteins
GTPases and their Regulation
Large G-proteins,...
Activation and Inactivation of G Proteins
Rab Proteins
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
The Ras Gene
Ras is a...

