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Biofuels

Biofuels

The microbial conversion of organic matter into biofuels holds potential as a renewable energy source. Among biofuel sources, microalgae are recognized as a highly efficient and adaptable feedstock for biodiesel production, owing to their rapid biomass accumulation, elevated lipid productivity, and capacity to proliferate in diverse aquatic systems, including freshwater, marine, and wastewater habitats. Unlike terrestrial crops, microalgae do not compete for land and can achieve significantly...

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Related Experiment Video

Updated: May 31, 2026

An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis

An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis

Published on: January 11, 2017

Microwave-Assisted Extraction of Hemp Seed Oil: Process Optimization for Enhancing Oil Yield and Bioactive Compound

Aymane Allay¹, Chaymae Benkirane¹, Abdessamad Ben Moumen¹

¹Laboratory of Agricultural Production Improvement, Biotechnology, and Environment, Faculty of Sciences, Mohammed I University, Oujda, Morocco.

International Journal of Food Science

|May 2, 2025

Summary

Optimizing microwave-assisted extraction of hemp seed oil enhances its stability and bioactive compound content. The study identified ideal conditions for maximizing oil yield and preserving valuable antioxidants, crucial for its nutritional quality.

Keywords:

hemp seed oil microwave-assisted extraction oxidative stability phenolic compounds tocopherols

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Published on: August 23, 2024

Area of Science:

Food Science and Technology
Biochemistry
Chemical Engineering

Background:

Hemp seed oil's high unsaturation leads to oxidation, reducing quality and nutritional value.
Incorporating natural antioxidants from hemp seeds can improve oil stability.
Understanding extraction process-composition interactions is key to optimizing hemp seed oil.

Purpose of the Study:

To investigate and optimize microwave-assisted extraction (MAE) of hemp seed oil.
To enrich the oil with bioactive compounds and enhance its oxidative stability.
To determine the effects of MAE parameters on oil yield, bioactive content, and quality indices.

Main Methods:

Response surface methodology (RSM) was employed to optimize MAE parameters.
Investigated factors included microwave power, ethanol cosolvent percentage, and extraction time.
Assessed responses: oil yield, total phenolic content (TPC), tocopherols, oxidative stability index (OSI), pigments, quality indices, and color.

Main Results:

Optimal conditions (800 W, 7.5% ethanol, 13.60 min) yielded 30.69% oil with high OSI (28.60 h).
The optimized oil was rich in TPC, tocopherols, carotenoids, and chlorophylls.
Microwave power and ethanol percentage significantly affected the phenolic profile, with specific conditions maximizing phenolic acids, hydrocinnamic acid amides, and lignanamides.

Conclusions:

MAE is an effective method for extracting high-quality hemp seed oil rich in bioactive compounds.
Optimized extraction parameters significantly improve oil yield, oxidative stability, and antioxidant content.
The study provides valuable insights for maximizing the stability and bioactive properties of hemp seed oil.