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Microbially Derived P=S and P=Se Bond Formation
Connor L Trotter1, Yuta Era1, Rory Gordon1,2
1Institute of Quantitative Biology, Biochemistry and Biotechnology, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3FF, U.K.
Abstract:
Microbial metabolism is a diverse and sustainable source of synthetic reagents that can be programmed for controlled and high-level production via synthetic biology. However, despite the chemical diversity of metabolism, the chemical utility of metabolites, and the available tools to control metabolic chemistry, there remain few examples of the use of cellular metabolites directly for chemical synthesis. Herein, we report that diverse bacteria perform P=S bond formation (Ph3P to Ph3PS) via central sulfur metabolism and nonenzymatic chemistry in vivo, which can also be applied to affect microbial P=Se bond formation (Ph3PSe). To the best of our knowledge, this is the first biochemical and genetic investigation of P=S bond formation in a microbial cell and the first use of microbial metabolites for P=Se bond formation in chemical synthesis.
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