Related Experiment Video
Updated: May 9, 2025
07:32
Preparation and Characterization of Individual and Multi-drug Loaded Physically Entrapped Polymeric Micelles
Published on: August 28, 2015
11.2K
Chitosan-modified ceftazidime loaded polyhydroxyalkanoates microparticles: preparation, characterization and
Anastasiya Murueva1,2, Natalia Zhila1,2, Alexey Dudaev1,2
1Institute of Biophysics SB RAS, Federal Research Center "Krasnoyarsk Science Center SB RAS", 50/50 Akademgorodok, Krasnoyarsk 660036, Russia.
ADMET & DMPK
|May 2, 2025
Summary
Chitosan-modified microparticles loaded with ceftazidime offer a promising drug delivery system for combating bacterial resistance. These microparticles demonstrate sustained drug release, good biocompatibility, and enhanced antibacterial activity against common pathogens.
Area of Science:
- Biomaterials Science
- Drug Delivery
- Antimicrobial Research
Background:
- Bacterial resistance necessitates novel antimicrobial strategies.
- Drug delivery systems can enhance the efficacy of existing antibiotics.
- Chitosan and polyhydroxyalkanoates (PHAs) offer potential as biocompatible carriers.
Purpose of the Study:
- To develop and characterize chitosan-modified ceftazidime-loaded poly(3-hydroxybutyrate-3-hydroxyvalerate-3-hydroxyhexanoate) (P(3HB-3HV-3HHх)) microparticles.
- To evaluate their potential as a topical drug delivery system for infectious skin diseases.
Main Methods:
- Microparticle preparation and characterization (size, PDI, zeta potential, encapsulation efficiency).
- In vitro drug release studies.
- Cytotoxicity, blood compatibility (hemolysis assay), and antibacterial activity assessments.
Main Results:
- Spherical microparticles (0.6–1.6 μm) with sustained ceftazidime release (Korsmeyer-Peppas and Higuchi models).
- Good cell viability and proliferation on HaCaT cells.
- Excellent blood compatibility and enhanced antibacterial efficacy against E. coli and St. aureus.
Conclusions:
- Chitosan-modified P(3HB-3HV-3HHх) microparticles serve as an effective depot for ceftazidime.
- These microparticles show promise for topical treatment of skin infections due to their biocompatibility and enhanced antimicrobial properties.
