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Updated: May 9, 2025
A High-Throughput Multiplexed Screening for Type 1 Diabetes, Celiac Diseases, and COVID-19
Published on: July 5, 2022
Screening Biomarkers and Risk Factors for COVID-19 Progression in a Border Population Between Brazil-Bolivia
Ana Maísa Passos-Silva1,2,3,4, Adrhyan Araújo1,2,3,4, Tárcio Peixoto Roca1,4,5
1Laboratório de Virologia Molecular, Fundação Oswaldo Cruz Rondônia - FIOCRUZ/RO, Porto Velho, Rondônia, Brazil.
Background:
The performance and genetic role in host response delineate investigative points of polymorphisms as potential biomarkers in viral infections.
Methods:
Thus, this research aimed to map biomarkers and risk factors in the severity of COVID-19 in individuals in Western Amazon (n = 243).
Results:
Patients aged 40 to 59 years showed an association with clinical progression (P = .003), also evidencing the relationship for individuals >60 years (P < .001), besides the non-vaccination influenced the pathology (P = .023). qPCR for human genotyping of the targets rs2070788, rs4702, rs76635825, rs540856718, rs35803318, rs12979860, and rs16899066, as well as for gene expression of ACE2, HLA-A, HLA-B, IFNL-3/2, IL-6, and TMPRSS2 was used. The rs12979860 (C > T) and rs2070788 (A > G) showed association among the analyzed groups (P < .05) with the allelic and genotypic frequency of rs12979860 (x 2 < 3.84) and evolutionary pointing of rs2070788G allele among infected people, including deaths.
Conclusion:
Gene expression showed high levels between the moderate and severe groups, with emphasis on TMPRSS2 and IL-6 genes that performed better. Thus, there is possibly an association regarding the role of the TMPRSS2 gene and rs2070788G, as well as age and IL-6 levels for COVID-19, pointing in parallel to the considerable influence of the vaccine on the SARS-CoV-2 pathway.
