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IFITM1 as a modulator of surfaceome dynamics and aggressive phenotype in cervical cancer cells

Nela Friedlová1, Lucie Bortlíková1, Lenka Dosedělová1

  • 1Research Centre for Applied Molecular Oncology, Masaryk Memorial Cancer Institute, 656 53 Brno, Czech Republic.

Oncology Reports
|May 2, 2025
PubMed

Insights

Interferon-induced transmembrane proteins (IFITMs) like IFITM1 are overexpressed in cervical cancer. This study reveals how IFITM1 alters the cancer cell surfaceome, impacting adhesion, migration, and immune interactions.

Area of Science:

  • Oncology
  • Cell Biology
  • Immunology

Background:

  • Interferon-induced transmembrane proteins (IFITMs) are implicated in cancer progression, but their precise roles and mechanisms, particularly in cervical carcinoma, are not fully elucidated.
  • Overexpression of IFITM proteins is observed in various cancer cells, suggesting a significant contribution to tumorigenesis.

Purpose of the Study:

  • To comprehensively characterize the influence of Interferon-induced transmembrane protein 1 (IFITM1) on the cervical cancer cell surfaceome.
  • To investigate the effects of interferon-gamma on protein expression and cell surface localization in the presence and absence of IFITM1.
  • To explore the functional consequences of IFITM1 and IFITM3 expression on cervical cancer cell behavior and immune cell interactions.

Main Methods:

  • Surface membrane protein isolation coupled with quantitative mass spectrometry to profile the cell surfaceome.
  • Reverse transcription-quantitative PCR (RT-qPCR) to analyze gene expression and differentiate transcriptional regulation from protein dynamics.
  • Functional assays to assess cell adhesion, migration, invasion, and interactions with immune cells.

Main Results:

  • IFITM1 significantly alters the composition of the cervical cancer cell surfaceome, identifying key regulated proteins involved in endocytosis, lysosomal transport, cell adhesion, antigen presentation, and immune responses.
  • Interferon-gamma modulates protein expression and cell surface exposure differently depending on IFITM1 presence.
  • Proteomic and gene expression data correlate with functional studies showing IFITM1 and IFITM3 impact on cervical cancer cell invasiveness and immune cell interactions.

Conclusions:

  • IFITM1 plays a crucial role in shaping the cervical cancer cell surfaceome, influencing critical cellular processes and interactions.
  • Understanding IFITM1's impact on the surfaceome provides insights into cervical cancer progression and potential therapeutic targets.
  • IFITM proteins influence cervical cancer cell malignancy and immune evasion, highlighting their importance in cancer immunology.
Keywords:
antigen presentationcervical cancerimmunosurveillanceinterferon‑induced transmembrane proteinsinvasionmass spectrometrymembrane proteinmigrationsurfaceome

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