Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Development of a spatial radiomics nomogram for predicting unfavorable outcomes after acute ischemic stroke: A multicenter study.

European journal of radiology·2026
Same author

Biomechanical mechanisms of early gait training on knee cartilage degeneration after anterior cruciate ligament reconstruction: a study protocol.

Frontiers in sports and active living·2026
Same author

Refined AI-ASPECTS with modified atlas and lesion-load thresholds: advancing acute ischemic stroke imaging and prognostic prediction.

BMC medicine·2026
Same author

Effects of the space environment on articular cartilage homeostasis: a review.

NPJ microgravity·2026
Same author

The Relationship between Glymphatic Dysfunction and Post-stroke Cognitive Impairment.

Current medical imaging·2026
Same author

Multimodal MRI-based nomogram integrating clinical-radiological, radiomic, and habitat features to discriminate solitary fibrous tumors from atypical meningiomas.

Scientific reports·2026

Related Experiment Video

Updated: May 9, 2025

Biofunctionalization of Magnetic Nanomaterials
06:40

Biofunctionalization of Magnetic Nanomaterials

Published on: July 16, 2020

2.5K

Layer-by-layer coating strategy to functionalize the magnetic nanoparticles for their multi-functionalization.

Jing Liu1, Ye Chen2, Hongjie Huang3

  • 1Department of Research, Shanghai University of Medicine and Health Sciences Affliated Zhoupu Hospital, The College of Medical Technology, Shanghai University of Medicine and Health Sciences, Shanghai, 201318, China.

Discover Nano
|May 2, 2025
PubMed
Summary
This summary is machine-generated.

This study presents a novel layer-by-layer coating method for magnetic nanoparticles (MNPs), enhancing their stability for electrochemical biosensors. The functionalized MNPs successfully combined redox and biological probes for potential disease diagnostics.

Keywords:
Bio-nano interactionElectroactive moleculesElectrochemical sensingLayer-by-layer (LbL) techniqueMagnetic nanoparticles

More Related Videos

Cell Labeling and Targeting with Superparamagnetic Iron Oxide Nanoparticles
08:26

Cell Labeling and Targeting with Superparamagnetic Iron Oxide Nanoparticles

Published on: October 19, 2015

12.0K
Fabrication of Magnetic Platforms for Micron-Scale Organization of Interconnected Neurons
09:54

Fabrication of Magnetic Platforms for Micron-Scale Organization of Interconnected Neurons

Published on: July 14, 2021

3.9K

Related Experiment Videos

Last Updated: May 9, 2025

Biofunctionalization of Magnetic Nanomaterials
06:40

Biofunctionalization of Magnetic Nanomaterials

Published on: July 16, 2020

2.5K
Cell Labeling and Targeting with Superparamagnetic Iron Oxide Nanoparticles
08:26

Cell Labeling and Targeting with Superparamagnetic Iron Oxide Nanoparticles

Published on: October 19, 2015

12.0K
Fabrication of Magnetic Platforms for Micron-Scale Organization of Interconnected Neurons
09:54

Fabrication of Magnetic Platforms for Micron-Scale Organization of Interconnected Neurons

Published on: July 14, 2021

3.9K

Area of Science:

  • Nanotechnology
  • Materials Science
  • Electrochemistry

Background:

  • Magnetic nanoparticles (MNPs) are promising for various applications but suffer from aggregation and instability upon surface modification.
  • Existing methods for MNP functionalization often compromise their stability and performance in biological environments.

Purpose of the Study:

  • To develop a facile and effective method for stabilizing and functionalizing MNPs for electrochemical biosensor applications.
  • To combine redox and bio-probes onto MNPs while maintaining their dispersion stability.

Main Methods:

  • A layer-by-layer (LbL) coating technique using polyallylamine hydrochloride (PAH) and poly(styrene sulfonic acid) sodium salt (PSS) was employed.
  • Redox molecules (ferrocene, anthraquinone, monocarboxymethylene blue) were anchored to MNPs in organic solvents, followed by LbL coating for protection.
  • Biomolecules (IgG) were attached to the outer polyelectrolyte shell without chemical cross-linking.

Main Results:

  • LbL-coated MNPs (MNPs@Fc, MNP@AQ, MNP@MB) maintained sizes between 10.5-12.4 nm.
  • Characterization by square wave voltammetry (SWV) showed distinct redox intensities for the functionalized MNPs.
  • An IgG binding efficiency of up to 78% was achieved, confirmed by SDS-PAGE analysis.

Conclusions:

  • The LbL coating technique provides a versatile and effective approach for functionalizing MNPs.
  • This method successfully integrates redox and biological properties onto MNPs, enhancing their stability and functionality.
  • The developed functionalized MNPs show significant potential for applications in disease diagnosis and point-of-care diagnostics.