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Structure characteristics and fermentation properties of konjac oligosaccharides from konjac peel prepared by
Qiqi Wang1, Songlin Liu2, Tianxiang Zhu3
1Key Laboratory of Traditional Chinese Medicine Resources and Traditional Chinese Medicine Formulas, Hubei University of Chinese Medicine, Huangjiahu West Road 16, Wuhan 430065, PR China; Hubei Shizhen Laboratory, Huayuanshan Road 4, Wuhan 430061, PR China; School of Pharmacy, Hubei University of Chinese Medicine, Huangjiahu West Road 16, Wuhan 430065, PR China.
Abstract:
Konjac peel is one of the main wastes in the processing of konjac. More effective green recycling solutions for konjac peel need to be developed. This study explored a green method for konjac oligosaccharide extraction from konjac peel, aiming to uncover the benefits of oligosaccharide preparation by Bacillus licheniformis (BJ2022) liquid-state fermentation. The extraction rate of fermented konjac oligosaccharides (FKO) by fermenting konjac peel in a 100 L fermentation tank reached 21.78 g/L, and the purity of the extracted oligosaccharides was 74.11 %. Pentasaccharides, hexasaccharides, and heptasaccharides were purified and separated from FKO. The oligosaccharides separated from FKO were composed of glucose and mannose connected by β-1,4-glycosidic bonds. The in vitro anaerobic fermentation experiments of gut microbiota and metabolomics analysis indicated that FKO had prebiotic activity in regulating gut microbiota and promoting the production of metabolites such as short-chain fatty acids. This study indicated that Bacillus licheniformis (BJ2022) one-step fermentation was a green and efficient konjac oligosaccharides production method.
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