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Updated: May 9, 2025

Qualitative Identification of Carboxylic Acids, Boronic Acids, and Amines Using Cruciform Fluorophores
Published on: August 19, 2013
Structure-function relationships in solvatochromic fluorophores targeting human and bovine carbonic anhydrases
Alyssa R Malto1, Radhika Mehta1, Abigail Hinojosa1
1Department of Chemistry, University of Texas at Austin, Austin, TX 78712, United States of America.
Abstract:
The development of reversible, selective, small molecule fluorescent probes displaying high fluorescence turn-on responses and tight binding affinity with specific metalloenzymes requires consideration of multiple factors. In this study, we report a library of fluorescent probes for two carbonic anhydrases: human carbonic anhydrase II (hCAII) and bovine carbonic anhydrase II (bCAII) and compare differences in fluorescence turn-on and binding affinity with molecular docking to gain insight into structure-function relationships. We analyze the roles of electrostatics, hydrophobics, and sterics in influencing the fluorescence response of the probes with hCAII and bCAII which have 80 % sequence identity but drastically different fluorescence turn-on values with amino(na)phthalimide-based fluorophores.
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