Jove
Visualize
Contact Us

Related Concept Videos

Chemiosmosis01:32

Chemiosmosis

96.1K
Oxidative phosphorylation is a highly efficient process that generates large amounts of adenosine triphosphate (ATP), the basic unit of energy that drives many cellular processes. Oxidative phosphorylation involves two processes— the electron transport chain and chemiosmosis.
Electron Transport Chain
The electron transport chain involves a series of protein complexes on the inner mitochondrial membrane that undergo a series of redox reactions. At the end of this chain, the electrons...
96.1K
The Supercomplexes in the Crista Membrane01:41

The Supercomplexes in the Crista Membrane

2.4K
The mitochondrial cristae membrane is the primary site for the oxidative phosphorylation (OXPHOS) process of energy conversion mediated through respiratory complexes I to V. These complexes have been widely studied for decades, and it has been proven that they form supramolecular structures called respiratory supercomplexes (SC). These higher-order complexes may be crucial in maintaining the biochemical structure and improving the physiological activity of the individual complexes while...
2.4K
Coupled Reactions01:17

Coupled Reactions

7.3K
Cellular processes such as building and breaking down complex molecules occur through stepwise chemical reactions. Some of these chemical reactions are spontaneous and release energy, whereas others require energy to proceed. Cells often couple the energy-releasing reaction with the energy-requiring one to carry out important cell functions. 
Energy in adenosine triphosphate or ATP molecules is easily accessible to do work. ATP powers the majority of energy-requiring cellular reactions....
7.3K
The Electron Transport Chain01:30

The Electron Transport Chain

15.7K
The electron transport chain or oxidative phosphorylation is an exothermic process in which free energy released during electron transfer reactions is coupled to ATP synthesis. This process is a significant source of energy in aerobic cells, and therefore inhibitors of the electron transport chain can be detrimental to the cell's metabolic processes.
Inhibitors of the electron transport chain
Rotenone, a widely used pesticide, prevents electron transfer from Fe-S cluster to ubiquinone or Q...
15.7K
Chemical Factors Affecting Respiration Centers01:31

Chemical Factors Affecting Respiration Centers

928
Chemical factors such as changing CO2, O2, and H+ levels in arterial blood play a critical role in influencing respiration depth and rates. These variations are detected by chemoreceptors—specialized sensors located in two primary body areas. Central chemoreceptors are found throughout the brain stem, including the ventrolateral medulla, while peripheral chemoreceptors are located in the aortic arch and carotid arteries.
CO2 has a potent influence on respiration and is strictly regulated....
928
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

145
Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
145

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The draft genome of Andean Rhodopseudomonas sp. strain AZUL predicts genome plasticity and adaptation to chemical homeostasis.

BMC microbiology·2022
Same author

Urine dilution with a synthetic wastewater (Syntho) boosts the electricity production in a bio-electrochemical system powered by un-pretreated human urine.

Bioelectrochemistry (Amsterdam, Netherlands)·2020
Same author

Biochemical capacitance of Geobacter sulfurreducens biofilms.

ChemSusChem·2015
Same author

Crystallographic orientation and electrode nature are key factors for electric current generation by Geobacter sulfurreducens.

Bioelectrochemistry (Amsterdam, Netherlands)·2014
Same author

C-type cytochromes wire electricity-producing bacteria to electrodes.

Angewandte Chemie (International ed. in English)·2008
Same journal

Host transcriptional signatures associated with disease tolerance and environmental persistence in a mosquito-microsporidian system.

Communications biology·2026
Same journal

Evolutionary dynamics of enlarged neo-sex chromosomes and novel pseudoautosomal regions in Sylvioidea songbirds.

Communications biology·2026
Same journal

NuSAP1 promotes spindle assembly in Trypanosoma brucei by bundling spindle microtubules.

Communications biology·2026
Same journal

Phenotypic and neuropeptidergic control of appetitive behavior in honey bees (Apis mellifera).

Communications biology·2026
Same journal

Fermentative iron reduction by a psychrotolerant Clostridium-dominant consortium enriched from Antarctic penguin-impacted soils.

Communications biology·2026
Same journal

Multilayer brain network analysis in mice reveals ketamine-induced reorganization of brain- wide fluctuations and gut-brain axis.

Communications biology·2026
See all related articles
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 9, 2025

High-Resolution Respirometry to Assess Bioenergetics in Cells and Tissues Using Chamber- and Plate-Based Respirometers
09:53

High-Resolution Respirometry to Assess Bioenergetics in Cells and Tissues Using Chamber- and Plate-Based Respirometers

Published on: October 26, 2021

4.5K

Decoupled respiration in electro-active bacteria.

Diego A Massazza1, Juan P Busalmen2, Hernán E Romeo3

  • 1Institute of Materials Science and Technology (INTEMA), University of Mar del Plata (Mar del Plata, Argentina) and National Research Council (CONICET, Argentina), Mar del Plata, Argentina. dmassazza@fi.mdp.edu.ar.

Communications Biology
|May 2, 2025
PubMed
Summary
This summary is machine-generated.

Electro-active bacteria, like Geobacter sulfurreducens, use energy dissipation mechanisms to prevent metabolic overload. The inner-membrane cytochrome CbcBA acts as a gate to decouple respiration from carbon assimilation when needed.

More Related Videos

Self-standing Electrochemical Set-up to Enrich Anode-respiring Bacteria On-site
05:29

Self-standing Electrochemical Set-up to Enrich Anode-respiring Bacteria On-site

Published on: July 24, 2018

7.6K
Characterizing Mediated Extracellular Electron Transfer in Lactic Acid Bacteria with a Three-Electrode, Two-Chamber Bioelectrochemical System
10:23

Characterizing Mediated Extracellular Electron Transfer in Lactic Acid Bacteria with a Three-Electrode, Two-Chamber Bioelectrochemical System

Published on: August 23, 2024

576

Related Experiment Videos

Last Updated: May 9, 2025

High-Resolution Respirometry to Assess Bioenergetics in Cells and Tissues Using Chamber- and Plate-Based Respirometers
09:53

High-Resolution Respirometry to Assess Bioenergetics in Cells and Tissues Using Chamber- and Plate-Based Respirometers

Published on: October 26, 2021

4.5K
Self-standing Electrochemical Set-up to Enrich Anode-respiring Bacteria On-site
05:29

Self-standing Electrochemical Set-up to Enrich Anode-respiring Bacteria On-site

Published on: July 24, 2018

7.6K
Characterizing Mediated Extracellular Electron Transfer in Lactic Acid Bacteria with a Three-Electrode, Two-Chamber Bioelectrochemical System
10:23

Characterizing Mediated Extracellular Electron Transfer in Lactic Acid Bacteria with a Three-Electrode, Two-Chamber Bioelectrochemical System

Published on: August 23, 2024

576

Area of Science:

  • Microbiology
  • Bioenergetics
  • Electrophysiology

Background:

  • Bacterial respiratory chains regulate electron transfer and proton translocation based on metabolic needs.
  • Energy dissipation mechanisms are known but not universally demonstrated across bacteria, especially electro-active ones.
  • Electro-active bacteria may possess unique responses to prevent metabolic overload.

Purpose of the Study:

  • To investigate if electro-active bacteria employ energy decoupling mechanisms in response to over-polarization.
  • To determine if the inner-membrane cytochrome CbcBA in Geobacter sulfurreducens functions as an energy dissipation gate.
  • To understand how this mechanism regulates cellular energy balance during electrode respiration.

Main Methods:

  • Electrochemical analyses were performed on electro-active Geobacter sulfurreducens.
  • Cellular respiration near the thermodynamic energetic limit was studied.
  • The role of inner-membrane cytochrome CbcBA in response to metabolic demands was investigated.

Main Results:

  • Geobacter sulfurreducens exhibits a response to over-polarization by triggering energy decoupling mechanisms.
  • The inner-membrane cytochrome CbcBA was identified as a key component in this response.
  • Cytochrome CbcBA acts as an energy dissipation gate, decoupling carbon assimilation from electrode respiration.

Conclusions:

  • Electro-active bacteria possess mechanisms to prevent metabolic overload, similar to other bacterial strains.
  • Cytochrome CbcBA plays a crucial role in regulating energy balance in Geobacter sulfurreducens.
  • This energy dissipation function of CbcBA allows bacteria to respire at the energetic limit while maintaining metabolic stability.