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Related Concept Videos

Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

251
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
251
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

272
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
272
IR Spectroscopy: Molecular Vibration Overview01:24

IR Spectroscopy: Molecular Vibration Overview

1.8K
When Infrared (IR) radiation passes through a covalently bonded molecule, the bonds transition from lower to higher vibrational levels. The fundamental vibrational motions that result in infrared absorption can be classified as stretching or bending vibrations.
Stretching vibrations are vibrational motions that occur along the bond line, changing the bond length or distance between two bonded atoms. They are further distinguished as symmetric or asymmetric. In symmetric stretching, the...
1.8K
IR Absorption Frequency: Delocalization01:04

IR Absorption Frequency: Delocalization

686
Electron delocalization refers to the distribution of electrons across multiple atoms within a molecule rather than being confined to a single atom or bond. This phenomenon is common in systems with conjugated bonds—structures where alternating single and double bonds allow π-electrons to move freely across the network. The movement of electrons stabilizes the molecule and can affect various chemical properties, including vibrational frequencies observed in IR spectroscopy.
In IR...
686
Applications of IR Spectroscopy: Overview01:11

Applications of IR Spectroscopy: Overview

429
The non-destructive nature and ability to provide valuable chemical information make IR spectroscopy a versatile technique with broad applications in various scientific and industrial fields. IR spectroscopy is commonly used to identify and characterize organic and inorganic compounds. It provides information about the functional groups present in a molecule and the bonding between atoms. This helps in the structural elucidation of compounds during organic synthesis, pharmaceutical research,...
429
Infrared (IR) Spectroscopy: Overview01:09

Infrared (IR) Spectroscopy: Overview

1.4K
When electromagnetic radiation passes through a material, atoms or molecules transition from a lower to a higher energy state by absorbing radiation corresponding to the energy difference between the two states. The absorption of infrared (IR) radiation causes transitions between vibrational energy levels in a molecule. Therefore, IR spectroscopy is a useful analytical tool for determining the molecular structure of molecules.
Different compounds display unique properties due to their...
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Updated: May 15, 2025

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional &#960;-conjugate Systems
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Colocalized Raman and IR Spectroscopies via Vibrational-Encoded Fluorescence for Comprehensive Vibrational Analysis.

Zhao-Dong Meng1, Tai-Rui Wu1, Li-Ling Zhou1

  • 1School of Electronic Science and Engineering, State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Fujian Key Laboratory of Ultrafast Laser Technology and Applications, IKKEM, Xiamen University, Xiamen 361005, China.

Journal of the American Chemical Society
|May 3, 2025
PubMed
Summary
This summary is machine-generated.

This study introduces vibrational-encoded fluorescence (VEF) to simultaneously detect Raman and infrared (IR) vibrational modes. This integrated approach enhances molecular analysis in complex chemical environments with ultrahigh sensitivity.

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Area of Science:

  • Molecular spectroscopy
  • Plasmonics
  • Nanophotonics

Background:

  • Vibrational spectroscopy (Raman and IR) offers molecular fingerprint information for sensing and diagnostics.
  • Complementary Raman and IR spectroscopies provide distinct insights but face challenges in simultaneous detection due to wavelength and sensitivity mismatches.
  • Existing methods struggle to capture complete vibrational data in complex chemical environments.

Purpose of the Study:

  • To develop an integrated approach for simultaneous detection of complementary Raman and IR vibrational modes.
  • To overcome the limitations of individual Raman and IR spectroscopies in complex chemical analysis.
  • To enable precise molecular vibrational information identification.

Main Methods:

  • Development of vibrational-encoded fluorescence (VEF) to encode Raman (Stokes) and IR (anti-Stokes) information into fluorescence.
  • Utilizing a dual-resonant microsphere-on-mirror plasmonic structure to bridge the waveband gap.
  • Employing hyperspectral colocalization imaging for spatial correlation analysis.

Main Results:

  • Simultaneous detection of complete vibrational modes in the visible spectrum achieved.
  • Ultrahigh sensitivity demonstrated, detecting down to approximately 100 molecules.
  • Detection efficiency improved by 8 orders of magnitude compared to unenhanced IR spectroscopy.
  • Spatial correlations between complementary vibrations confirmed via imaging.

Conclusions:

  • The VEF approach successfully integrates complementary vibrational information.
  • This method offers unprecedented sensitivity and efficiency for molecular analysis.
  • Creates new opportunities for precise molecular identification in complex chemical settings.