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Related Experiment Videos

Developmental variability within and between mouse expanding blastocysts and their ICMs.

J C Chisholm, M H Johnson, P D Warren

    Journal of Embryology and Experimental Morphology
    |April 1, 1985
    PubMed
    Summary

    Mouse blastocyst development shows significant heterogeneity. Even when synchronized, cell numbers, DNA content, and developmental potential vary widely, indicating complex cell cycle dynamics and potential restrictions in inner cell mass development.

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    Area of Science:

    • Developmental Biology
    • Cell Biology
    • Embryology

    Background:

    • Mouse blastocyst development is crucial for understanding early embryogenesis.
    • Significant heterogeneity exists among embryos, complicating research.
    • Synchronizing embryos is a strategy to mitigate developmental variability.

    Purpose of the Study:

    • To investigate developmental heterogeneity in mouse blastocysts.
    • To analyze cell number, size, DNA content, and developmental potential.
    • To understand cell cycling relative to blastocoel formation and inner cell mass development.

    Main Methods:

    • Synchronizing mouse embryos at the initial blastocoel formation stage.
    • Quantifying inside and outside cell numbers and inside cell size.

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  • Assessing nuclear DNA content and developmental potential of embryos.

    • Main Results:

    • Wide heterogeneity observed in cell number ratios and cell cycling among synchronized embryos.
    • Blastocoel formation appears to initiate when outer cells are in their sixth cell cycle.
    • Outer cells generally exhibit faster cell cycling than inner cells.

    Conclusions:

    • Mouse blastocyst development exhibits considerable heterogeneity even after synchronization.
    • Cell cycling patterns suggest outer cells are slightly more advanced than inner cells.
    • Evidence suggests developmental potential restriction occurs at different times within individual inner cell masses (ICMs).