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Related Experiment Video

Updated: May 9, 2025

Assay for Pathogen-Associated Molecular Pattern PAMP-Triggered Immunity PTI in Plants

Assay for Pathogen-Associated Molecular Pattern PAMP-Triggered Immunity PTI in Plants

Published on: September 9, 2009

Protocol for probing candidate effector activity using the Prf-based plant cell death phenotype.

Jiaxing Liu¹, Sonia Irigoyen¹, Kranthi K Mandadi²

¹Texas A&M AgriLife Research and Extension Center, 2415 East US Highway 83, Weslaco, TX 78596, USA.

|May 3, 2025

Summary

This study introduces a new method to test plant pathogen effector proteins. The protocol uses a plant cell death assay to identify effectors that interfere with plant immunity.

Keywords:

Genetics Molecular biology Plant sciences

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Last Updated: May 9, 2025

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Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens

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Author Spotlight: A Streamlined Approach to Studying Cell Death Initiation in Hypersensitive Response

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Published on: November 10, 2023

Area of Science:

Plant Pathology
Molecular Plant-Microbe Interactions
Plant Immunity

Background:

Understanding plant-microbe interactions requires identifying and characterizing pathogen effector proteins.
Effector proteins play crucial roles in modulating plant immune responses and disease development.

Purpose of the Study:

To present a novel protocol for probing the activity of candidate effector proteins.
To enable functional characterization of effectors using a plant cell death phenotype.

Main Methods:

Co-infiltration of plant leaves with a resistance protein (Prf) and candidate effector proteins.
Visual assessment of the plant cell death phenotype as a readout for effector activity.
Utilizing the Prf-based cell death assay for screening effector functions.

Main Results:

The protocol successfully enables the detection of effector activity.
Identified candidate effectors that suppress programmed cell death.
Demonstrated the utility of the method in studying effectors that interfere with plant immune responses.

Conclusions:

The developed protocol provides an efficient means to functionally characterize plant pathogen effectors.
This method aids in understanding how effectors manipulate plant immunity.
Facilitates the screening of effectors involved in suppressing plant programmed cell death.