Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Therapeutic Drug Monitoring: Affecting Factors01:29

Therapeutic Drug Monitoring: Affecting Factors

Therapeutic Drug Monitoring (TDM) is the clinical practice of measuring specific drug levels in a patient's blood or body tissues to manage and optimize therapy. TDM is crucial for drugs with narrow therapeutic windows, like warfarin and phenytoin, where incorrect doses can lead to treatment failure or severe side effects. This monitoring ensures the dosage administered is within a safe and effective range. The factors affecting therapeutic drug monitoring include:Patient-Specific Factors:a.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Regional surveillance of non-A-F Salmonella enterica reveals diverse risk profiles, with global genomic insights from Salmonella Worthington.

Food research international (Ottawa, Ont.)·2026
Same author

Integrating bulk RNA-seq and ScRNA-seq to identify manganese metabolism-related subtypes and immunoregulatory mechanisms in liver hepatocellular carcinoma.

Open life sciences·2026
Same author

The Molecular Biological Characteristics of <i>Vibrio vulnificus</i> Isolated from Wenzhou, China.

Foodborne pathogens and disease·2026
Same author

Bile acid dependent attenuation of toxin mediated disease is independent of colonization resistance against <i>C. difficile</i>.

bioRxiv : the preprint server for biology·2026
Same author

Successful fecal microbiota transplants in post-antibiotic treated recurrent <i>Clostridioides difficile</i> patients induce acylcarnitine and sphingolipid lipidomic shifts.

Gut microbes reports·2026
Same author

Highly Sensitive Control Study of PD Archimedean Antenna Based on Rotating Unit Reflective Metasurface.

Micromachines·2026

Related Experiment Video

Updated: May 12, 2026

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids
06:53

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

Published on: June 8, 2019

8.6K

Plasma DNMT1 Activity for Assessing Tumor Burden and Predicting Neoadjuvant Therapy Response in Breast Cancer.

Yingran Wang1, Guozhi Zhang2, Zhizhao Zhang2

  • 1Department of Clinical Laboratory Medicine, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, 400038, P. R. China.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|May 3, 2025
PubMed
Summary

A new method, DNMT1 Identification by Variable Activity (DIVA), accurately detects DNA methyltransferase 1 (DNMT1) activity in breast cancer patients' blood. This technique aids in evaluating tumor burden and treatment response.

Keywords:
breast cancermodularized tandem reactionneoadjuvant therapy response predictionplasma DNMT1tumor burden assessment

More Related Videos

Testing Targeted Therapies in Cancer using Structural DNA Alteration Analysis and Patient-Derived Xenografts
10:27

Testing Targeted Therapies in Cancer using Structural DNA Alteration Analysis and Patient-Derived Xenografts

Published on: July 25, 2020

7.1K
Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors
06:07

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

Published on: August 5, 2022

2.5K

Related Experiment Videos

Last Updated: May 12, 2026

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids
06:53

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

Published on: June 8, 2019

8.6K
Testing Targeted Therapies in Cancer using Structural DNA Alteration Analysis and Patient-Derived Xenografts
10:27

Testing Targeted Therapies in Cancer using Structural DNA Alteration Analysis and Patient-Derived Xenografts

Published on: July 25, 2020

7.1K
Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors
06:07

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

Published on: August 5, 2022

2.5K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Oncology

Background:

  • DNA methylation, regulated by DNA methyltransferases (DNMTs), is crucial for cellular function.
  • DNMT1 is a key target for cancer research, but its activity is difficult to measure in peripheral blood.

Purpose of the Study:

  • To develop a sensitive method for quantifying DNMT1 activity in peripheral blood.
  • To evaluate the utility of this method for breast cancer detection and treatment monitoring.

Main Methods:

  • Development of the DNMT1 Identification by Variable Activity (DIVA) reaction system.
  • Application of DIVA to analyze 271 clinical samples from breast cancer patients (Stages I-IV).
  • Validation of DIVA in assessing prognosis for 22 patients undergoing neoadjuvant therapy.

Main Results:

  • DIVA achieves high sensitivity, detecting DNMT1 at levels as low as 10⁻⁷ U mL⁻¹.
  • The method successfully evaluated tumor burden across different breast cancer stages.
  • DIVA results showed good consistency with ultrasound imaging for neoadjuvant therapy assessment.

Conclusions:

  • DIVA is a sensitive, cost-effective, and rapid assay for DNMT1 activity in peripheral blood.
  • DIVA shows potential as an auxiliary tool for early breast cancer detection.
  • DIVA can be used to evaluate the effectiveness of neoadjuvant therapy in breast cancer patients.