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Related Experiment Video

Updated: May 9, 2025

Fabricating Highly Open Porous Microspheres HOPMs via Microfluidic Technology
05:21

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3D-Printed Microfluidic Platform for Creating Porous Nanofibrous Microspheres to Regulate Cell Response and Enhance

Donghee Lee1, Huy Quang Tran1, Navatha Shree Sharma1

  • 1Department of Surgery-Transplant and Mary & Dick Holland Regenerative Medicine Program, University of Nebraska Medical Center, Omaha, NE, 68198, USA.

Small (Weinheim an Der Bergstrasse, Germany)
|May 3, 2025
PubMed
Summary

A new 3D-printed microfluidic method fabricates scalable porous nanofibrous microspheres (PNMs) for tissue regeneration. These PNMs enhance bone and blood vessel formation and reduce inflammation, showing great potential for wound healing applications.

Keywords:
3D‐printed microfluidic platformbioactive molecule functionalizationporous nanofibrous microspheresregulation of cell responsetissue regeneration

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Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Microfluidics

Background:

  • Porous nanofibrous microspheres (PNMs) are promising for tissue regeneration due to their biomimetic properties and injectability.
  • Existing fabrication methods like self-assembly and co-axial electrospray have limitations in compositional versatility and scalability.
  • There is a need for scalable and controllable methods to produce PNMs for diverse regenerative applications.

Purpose of the Study:

  • To develop a scalable and controllable fabrication platform for porous nanofibrous microspheres (PNMs).
  • To functionalize PNMs with bioactive molecules to enhance their regenerative capabilities.
  • To evaluate the in vivo performance of PNMs for tissue integration and regeneration.

Main Methods:

  • Fabrication of PNMs using a 3D-printed microfluidic platform for precise control over size, pore architecture, and morphology.
  • Functionalization of PNMs with bioactive molecules via UV crosslinking.
  • In vitro assessment of PNM effects on osteogenesis (hBMSCs), angiogenesis (HUVECs), and inflammation (macrophages).
  • In vivo subcutaneous implantation in rats to evaluate cell infiltration, fibrosis, and tissue integration.

Main Results:

  • The microfluidic platform enabled large-scale PNM production with controlled characteristics.
  • Functionalized PNMs promoted osteogenesis, angiogenesis, and exhibited anti-inflammatory effects.
  • In vivo studies showed PNMs supported cell infiltration, minimized fibrosis, and integrated with host tissue within 14 days.

Conclusions:

  • 3D-printed microfluidics offer a scalable and versatile approach for fabricating customizable PNMs.
  • Functionalized PNMs demonstrate significant potential for promoting tissue regeneration and wound healing.
  • PNMs are suitable for applications as injectable carriers or powders in regenerative medicine.