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Cerebrospinal Fluid

Cerebrospinal Fluid

Cerebrospinal fluid (CSF) is a colorless liquid that flows around the brain and the spinal cord, playing a vital role in the protection, support, and overall function of the central nervous system (CNS). CSF production, circulation, and absorption are tightly regulated processes essential for the brain and spinal cord to function properly.
CSF Production
CSF is produced mainly in the choroid plexus, a network of capillaries and ependymal cells located within the ventricular system of the brain....

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Related Experiment Video

Updated: May 9, 2025

Detection and Quantification of Calcitonin Gene-Related Peptide CGRP in Human Plasma Using a Modified Enzyme-Linked Immunosorbent Assay

Detection and Quantification of Calcitonin Gene-Related Peptide CGRP in Human Plasma Using a Modified Enzyme-Linked Immunosorbent Assay

Published on: June 16, 2023

Cerebrospinal Fluid Concentrations of Calcitonin Gene Related Peptide in Dogs With Chiari-Like Malformation.

John D Macri¹, Courtney Sparks¹, Zachary Anderson¹

¹Department of Clinical Sciences, North Carolina State University, Raleigh, North Carolina, USA.

Journal of Veterinary Internal Medicine

|May 3, 2025

Summary

This summary is machine-generated.

Calcitonin Gene-Related Peptide (CGRP) levels in cerebrospinal fluid were higher in painful Cavalier King Charles Spaniels (CKCS). This suggests CGRP may contribute to neuropathic pain in dogs with Chiari-like malformation-syringomyelia (CMSM).

Keywords:

cavalier king Charles spaniel migraine neuropathic pain neuropeptides syringomyelia

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Ex Vivo Release of Calcitonin Gene-Related Peptide from the Trigeminovascular System in Rodents

Ex Vivo Release of Calcitonin Gene-Related Peptide from the Trigeminovascular System in Rodents

Published on: May 16, 2022

Area of Science:

Veterinary Neurology
Pain Research
Biomarker Discovery

Background:

Calcitonin Gene-Related Peptide (CGRP) is a known biomarker for neuropathic pain in humans.
Its role in Cavalier King Charles Spaniels (CKCS) with Chiari-like malformation (CM) and associated pain has not been investigated.
Chiari-like malformation-syringomyelia (CMSM) is a common neurological condition in CKCS causing pain.

Purpose of the Study:

To investigate cerebrospinal fluid (CSF) CGRP concentrations in CKCS.
To compare CGRP levels between CKCS with and without clinical signs of CMSM.
To determine if CSF CGRP correlates with pain and syringomyelia severity.

Main Methods:

A prospective study involving 29 CKCS, including those with and without clinical signs of CMSM.
Quantification of pain and scratching, MRI of the brain and spinal cord, and lumbar CSF collection.
CSF CGRP concentration measured using ELISA, with statistical comparison between groups and correlation analysis.

Main Results:

CSF CGRP concentration was significantly higher in painful CKCS compared to non-painful dogs (p=0.0124).
No significant difference in CGRP concentration was found between dogs with and without syringomyelia (SM) (p=0.305).
Pain score and SM diameter did not significantly correlate with CGRP concentration.

Conclusions:

Elevated CSF CGRP is associated with pain in CKCS, irrespective of syringomyelia presence.
CGRP may play a role in the neuropathic pain experienced by CKCS with CMSM.
CGRP emerges as a potential therapeutic target for managing pain in this canine population.