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Updated: May 9, 2025

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Rab5if is a potential therapeutic target of NSCLC

Linjuan Lu1, Lixiu Chen1, Feng Gao1

  • 1Department of Respiratory and Critical Care Medicine, The Affiliated Zhangjiagang Hospital of Soochow University, Suzhou, China.

Cancer Genetics
|May 3, 2025
PubMed
Abstract

Insights

Rab5if promotes non-small cell lung cancer (NSCLC) growth and migration. Targeting Rab5if may offer a new therapeutic strategy for NSCLC, as inhibiting its expression reduced tumor progression in vitro and in vivo.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Non-small cell lung cancer (NSCLC) remains a leading cause of cancer mortality globally.
  • High mortality rates are attributed to disease progression and acquired drug resistance.
  • Novel therapeutic targets are crucial for improving NSCLC treatment outcomes.

Purpose of the Study:

  • To investigate the role of Rab5if in the development and progression of NSCLC.
  • To explore the potential of Rab5if as a therapeutic target for NSCLC treatment.

Main Methods:

  • Construction of Rab5if-overexpressing and knockdown NSCLC cell lines using lentivirus.
  • In vitro cellular assays to assess proliferation, migration, and mitochondrial function.
  • Western blot analysis to elucidate the underlying molecular mechanisms.
  • In vivo xenograft studies in nude mice to validate in vitro findings.

Main Results:

  • Rab5if mRNA and protein levels were elevated in NSCLC tissues and cell lines.
  • Rab5if knockdown significantly inhibited NSCLC cell proliferation, migration, and mitochondrial function.
  • Rab5if overexpression promoted NSCLC cell proliferation and migration.
  • The AKT-mTOR pathway was identified as the key mechanism mediating Rab5if's effects.
  • In vivo studies confirmed that Rab5if knockdown inhibited tumor growth, while overexpression accelerated it.

Conclusions:

  • Rab5if plays a significant role in NSCLC progression.
  • Rab5if is a potential novel therapeutic target for NSCLC treatment.
Keywords:
AKT-mTORMolecular targeted therapyNon-small cell lung cancerRab5if

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