Related Experiment Video

Updated: Jun 13, 2025

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

Published on: September 25, 2019

Generation of a gene-corrected isogenic human iPS cell line (CSUASOi006-A-2) from a retinitis pigmentosa patient

Hang Chen¹, Yuqin Liang², Yuan Liang³

¹Department of Ophthalmology, The First Affiliated Hospital of Jinan University, Guangzhou 510632, China; Aier Eye Institute, Changsha 410015, China.

Stem Cell Research

|May 3, 2025

Abstract:

Retinitis pigmentosa (RP) is a heterogeneous group of hereditary eye disorders characterized by a progressive degeneration of the light-sensing photoreceptor cells in the retina. Currently, there are no effective treatments. In a previous study, we generated a human induced pluripotent stem (iPS) cell line (CSUASOi006-A) from an RP patient carrying a PRPF8 (c.C5792T) mutation. In this study, we corrected the c.5792C > T mutation in the PRPF8 gene using CRISPR/Cas9 technology and generated an isogenic control cell line (CSUASOi006-A-2). This provides an important cellular resource for RP research.

More Related Videos

Generation of Retinal Organoids from Healthy and Retinal Disease-Specific Human-Induced Pluripotent Stem Cells

Generation of Retinal Organoids from Healthy and Retinal Disease-Specific Human-Induced Pluripotent Stem Cells

Published on: December 9, 2022

Genome Editing and Directed Differentiation of hPSCs for Interrogating Lineage Determinants in Human Pancreatic Development

Genome Editing and Directed Differentiation of hPSCs for Interrogating Lineage Determinants in Human Pancreatic Development

Published on: March 5, 2017

Related Concept Videos

iPS Cell Differentiation

iPS Cell Differentiation

The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.

Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...

Articles linked to this work by shared authors, journal, and citation graph.

Applications of Machine Learning in the Research of Heavy Metal(loid)s-Related Risk: A Scoping Review of Methodology.

Toxics·2026

Generation of iPSC-derived limbal epithelial stem cells via a transparent defined protocol optimized by stromal cell co-culture.

Experimental eye research·2026

Nfe2l2-knockout mouse model exhibits oxidative stress-associated stromal degeneration and inflammatory activation resembling keratoconus pathology.

PeerJ·2026

Controls of disinfection byproducts and biotoxicity in chlorinated post-denitrifying effluents: Optimization of the carbon-to-nitrogen ratio and hydraulic retention time.

Eco-Environment & Health·2026

Generation of a human iPSC line (CSUASOi016-A) modeling X-linked retinoschisis by introducing the RS1 c.214G>A mutation using CRISPR/Cas9.

Stem cell research·2026

Generation of iPSC line CSUASOi015-A from peripheral blood mononuclear cells of an autosomal dominant retinitis pigmentosa patient with a heterozygous deletion of exons 2-8 in PRPF31.

Stem cell research·2026

See all related articles