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Decellularised amniotic membrane-TDSCs composite promotes Achilles tendon healing
Xufeng Mao1, Yulong Wang1, Xiqian Zhang1
1Department of Orthopaedic Surgery, The First Affiliated Hospital of Ningbo University, Ningbo, Zhejiang, People's Republic of China.
Scientific Reports
|May 3, 2025
Summary
A novel biomimetic membrane material, dAM-TDSCs, significantly enhances Achilles tendon healing by promoting cell growth and differentiation. This discovery offers a promising new therapeutic direction for tendon injuries.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Orthopedic Surgery
Background:
- Achilles tendon injuries often result in adhesions and poor healing, lacking effective treatment solutions.
- Current therapeutic options for Achilles tendon rupture have limitations in promoting complete and functional regeneration.
Purpose of the Study:
- To evaluate the efficacy of a biomimetic peritendinous membrane (dAM) in improving Achilles tendon healing.
- To investigate the potential of dAM combined with tendon-derived stem cells (TDSCs) for enhanced tendon regeneration.
Main Methods:
- In vitro assessment of TDSC adhesion, proliferation, and tenogenic differentiation on dAM.
- In vivo evaluation of dAM + TDSCs in a rat Achilles tendon rupture model.
- Omics analysis (mRNA transcriptome and proteomic) to elucidate the underlying repair mechanisms, including the ERK signaling pathway.
Main Results:
- dAM significantly enhanced TDSC adhesion, proliferation, and differentiation in vitro.
- Combined dAM + TDSCs implantation accelerated tendon regeneration in vivo.
- Omics analysis identified extracellular matrix-related genes and proteins and implicated the ERK pathway in tendon repair.
Conclusions:
- The dAM-TDSCs composite biomimetic membrane effectively promotes Achilles tendon healing.
- This biomimetic material presents a novel and promising therapeutic strategy for Achilles tendon injuries.
Keywords:
Achilles tendon ruptureAmniotic membraneBiomimetic peritendinous membraneTendon derived stem cellsMore Related Videos
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