Related Experiment Video

Updated: May 9, 2025

An Orthotopic Mouse Model of Anaplastic Thyroid Carcinoma
07:01

An Orthotopic Mouse Model of Anaplastic Thyroid Carcinoma

Published on: April 17, 2013

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Regulation of iodide transportation by a plant secondary metabolite in SW1736 anaplastic thyroid cancer cell line

Shabnam Heydarzadeh1, Ali Asghar Moshtaghie2, Maryam Daneshpour1

  • 1Cellular and Molecular Endocrine Research Center, Research Institute for Endocrine Molecular Biology, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Discover Oncology
|May 3, 2025
PubMed
Abstract

Insights

Genistein treatment increases Sodium iodide symporter (NIS) expression and iodide uptake in anaplastic thyroid cancer cells. This suggests potential redifferentiation, though not necessarily increased sensitivity to radioiodine therapy.

Area of Science:

  • Oncology
  • Molecular Biology
  • Nutraceutical Research

Background:

  • Anaplastic thyroid carcinoma (ATC) is highly aggressive and resistant to radioiodine therapy due to impaired iodine metabolism.
  • Understanding mechanisms to re-sensitize ATC to radioiodine is crucial for improving treatment outcomes.

Purpose of the Study:

  • To investigate the effect of Genistein, a plant metabolite, on Sodium iodide symporter (NIS) expression and iodide uptake in SW1736 anaplastic thyroid cancer cells.
  • To assess Genistein's role in potentially modulating ATC cell behavior towards a more differentiated state.

Main Methods:

  • Cell viability assessed via MTT assay and apoptosis via flow cytometry.
  • Iodide uptake measured using a spectrophotometric assay.
  • NIS mRNA and protein levels determined by QRT-PCR and Western blotting, respectively.

Main Results:

  • Genistein demonstrated time- and dose-dependent inhibition of SW1736 cell growth.
  • Genistein significantly upregulated NIS mRNA and protein expression in SW1736 cells (p < 0.05 and p < 0.01, respectively).
  • Genistein treatment markedly increased iodide uptake in SW1736 cells (p < 0.001).

Conclusions:

  • Genistein treatment appears to induce a redifferentiation-like effect in ATC cells by upregulating NIS expression and enhancing iodide uptake.
  • Despite increased iodide uptake, this observed redifferentiation did not translate to increased sensitivity to radioactive iodine treatment.
Keywords:
Anaplastic thyroid cancerGenisteinIodide uptakePlant secondary metaboliteSodium iodide symporter

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