Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

New Polyene Pregnane Steroids From the Stems of Nerium oleander: Structural Characterization and In Vitro Anti-Inflammatory Evaluation.

Chemistry & biodiversity·2026
Same author

Strong yet highly conductive liquid metal composite film constructed via friction induced in-situ synthesis.

Nature communications·2026
Same author

Transcutaneous Electrical Acupoints Stimulation Improves Spontaneous Voiding Recovery After Laparoscopic Cholecystectomy: A Randomized Clinical Trial.

World journal of surgery·2023
Same author

Effect of grazing disturbance on floral display, pollen limitation and plant pollination efficiency in the desert steppe.

BMC plant biology·2022
Same author

Evaluation of fertilization capability of frozen-thawed completely immotile spermatozoa collected from a white bengal tiger after interspecific ICSI with bovine oocytes.

Animal reproduction·2022
Same author

Effects of recipient oocyte source, number of transferred embryos and season on somatic cell nuclear transfer efficiency in sheep.

Reproduction in domestic animals = Zuchthygiene·2019

Related Experiment Video

Updated: May 9, 2025

Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay
08:00

Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay

Published on: June 16, 2021

4.3K

Transcriptome Analysis of Individual Ovine Oocytes and Preimplantation Embryos From In Vitro Fertilization Source.

Hai-Jun Liu1, Xiang-Yun Li2, Cheng-Jun Huang3

  • 1Institute of Animal Science and Veterinary Medicine, Tianjin Academy of Agriculture Sciences, Tianjin, China.

Molecular Reproduction and Development
|May 4, 2025
PubMed
Summary

This study reveals sheep zygotic genome activation occurs at the 8-cell stage, detailing gene expression changes during early embryo development. It provides a comprehensive transcriptome landscape for sheep oocytes and embryos.

Keywords:
RNA sequencingin vitro fertilization embryossheeptranscriptome analysis

More Related Videos

Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform
09:04

Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform

Published on: January 30, 2017

7.7K
Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes
12:11

Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes

Published on: May 11, 2017

10.9K

Related Experiment Videos

Last Updated: May 9, 2025

Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay
08:00

Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay

Published on: June 16, 2021

4.3K
Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform
09:04

Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform

Published on: January 30, 2017

7.7K
Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes
12:11

Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes

Published on: May 11, 2017

10.9K

Area of Science:

  • Reproductive Biology
  • Genomics
  • Developmental Biology

Background:

  • Understanding early embryonic development is crucial for improving reproductive technologies.
  • Sheep are an important livestock model for studying mammalian embryogenesis.

Purpose of the Study:

  • To comprehensively analyze the transcriptional landscape of sheep preimplantation embryos.
  • To identify key developmental stages and gene expression patterns during early embryogenesis.

Main Methods:

  • RNA sequencing was performed on eight developmental stages of in vitro fertilized sheep embryos, from germinal vesicle oocytes to blastocysts.
  • Cluster analysis and differential gene expression analysis were used to identify stage-specific transcriptomes.
  • Gene Ontology and KEGG pathway analyses were conducted to understand the functional significance of differentially expressed genes.

Main Results:

  • Between 12,744 and 15,539 genes were detected across developmental stages.
  • A significant increase in differentially expressed genes (DEGs) between the 4- and 8-cell stages suggests zygotic genome activation at the 8-cell stage.
  • Oxidative phosphorylation was the most enriched pathway, and Zf-C2H2, bHLH, Homeobox, Others, and HMG were the top transcription factor families identified.

Conclusions:

  • This study provides the first comprehensive transcriptional profiling of sheep preimplantation development.
  • The findings offer insights into the molecular mechanisms governing early sheep embryogenesis and zygotic genome activation.
  • The data establishes a valuable resource for future research in sheep reproductive biology and genomics.