Validated HPLC-UV Detection Method for Cefiderocol Quantification in a Critical Patient Receiving Fetcroja and...

A simple, rapid, and sensitive HPLC-UV method for cefiderocol quantification in plasma was developed to study the pharmacokinetic profile in a critical patient receiving Fetcroja and treated with a renal replacement therapy. After a quick deproteinization step by an effective precipitation, a chromatographic separation was performed with a C18 column and a mobile phase composed by acetonitrile and phosphate buffer at pH 3.50, delivered by gradient elution at a flow rate of 1.0 mL min^-1. The UV detector was set at 260 nm. Metronidazole was used as an internal standard. A total run time analysis of 15 min was obtained. The method was validated according to the standard guidelines and applied to study the pharmacokinetics of cefiderocol in a critical patient. Total run time analysis obtained was shorter than previously HPLC reported methods, being useful for therapeutic drug monitoring or pharmacokinetic profile research.