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Updated: May 9, 2025

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Published on: June 21, 2021
Towards site-specific manipulation in cysteine-mediated redox signaling
Jing Yang1,2,3
1Guangzhou National Laboratory, Guangzhou International Bio-Island Guangzhou China yangjing@ncpsb.org.cn.
Abstract:
Cysteine sulfenic acid (SOH) modifications are pivotal in redox signaling, yet establishing their causal biological roles remains challenging due to methodological limitations. Traditional approaches often lack precision or disrupt non-redox cysteine functions. This perspective highlights two innovative chemical biology strategies to address these challenges: (1) integrating bioorthogonal cleavage chemistry with genetic code expansion for site-specific SOH incorporation in proteins of interest, enabling controlled activation of redox events, and (2) developing redox-targeted covalent inhibitors (TCIs) to selectively block SOH modifications. By bridging technological innovation with mechanistic inquiry, these strategies not only help elucidate SOH-mediated signaling networks for a better understanding of redox biology, but also hold therapeutic promise for precise redox medicine.
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