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High Throughput Single-cell and Multiple-cell Micro-encapsulation

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Published on: June 15, 2012

Maximizing doses from multi-dose vaccine vials using the air bubble trapping technique.

Bethany C Yang¹, Kathleen M Sanchez¹, Kim M Moore¹

¹Vaccine Preventable Disease Control Program, Department of Public Health, Los Angeles County, Los Angeles, CA, USA.

Clinical and Experimental Vaccine Research

|May 5, 2025

Summary

The air bubble trapping technique (ABTT) minimizes vaccine waste from vials, reducing dead volume loss. While effective, ABTT requires slightly longer preparation times for vaccine doses.

Keywords:

Administration and dosage Hypodermic needle Syringes Vaccination Vaccine

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Area of Science:

Public Health
Vaccinology
Pharmaceutical Sciences

Background:

Vaccine shortages pose significant public health challenges.
Strategies like fractional dosing and adjuvants are used to conserve vaccine doses.
Reducing vaccine wastage is crucial for efficient vaccine distribution.

Purpose of the Study:

To evaluate the effectiveness of the air bubble trapping technique (ABTT) in minimizing vaccine wastage.
To compare ABTT with standard vaccine preparation methods.
To assess the impact of ABTT on dose extraction from multi-dose vials.

Main Methods:

The study compared ABTT with standard methods using three different syringe types.
Healthcare workers prepared 0.1 mL and 0.5 mL saline doses.
Doses were prepared both with and without the air bubble trapping technique.

Main Results:

ABTT reduced volume loss by 8.6% for 0.1 mL doses and 2.9% for 0.5 mL doses.
Preparation times increased with ABTT: 30.63 seconds for 0.1 mL and 32.95 seconds for 0.5 mL doses.
ABTT demonstrated consistency across various syringe types and user experience levels.

Conclusions:

The air bubble trapping technique effectively minimizes vaccine volume loss from multi-dose vials.
ABTT is a practical technique for integration into vaccination workflows.
While ABTT increases preparation time, its dose-saving benefits are significant.