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Related Experiment Video

Updated: May 9, 2025

Low-cost Custom Fabrication and Mode-locked Operation of an All-normal-dispersion Femtosecond Fiber Laser for Multiphoton Microscopy
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Multiphoton microscopy at a microwatt level via gain-managed nonlinear amplification and pulse-picking.

Katarzyna Kunio1, Grzegorz Soboń1, Jakub Bogusławski1

  • 1Laser & Fiber Electronics Group, Faculty of Electronics, Photonics and Microsystems, Wrocław University of Science and Technology, Wybrzeże Wyspiańskiego 27, 50-370 Wrocław, Poland.

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Summary

We developed a compact fiber laser system for multiphoton microscopy. This versatile laser enables high-resolution imaging at exceptionally low excitation powers, enhancing experimental flexibility and advancing biological research.

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Area of Science:

  • Biophotonics and Imaging
  • Laser Physics and Engineering

Background:

  • Multiphoton microscopy requires advanced laser systems for high-resolution imaging.
  • Existing systems can be bulky, complex, and require high excitation powers.
  • Need for versatile, compact, and low-power laser sources for broader accessibility.

Purpose of the Study:

  • To introduce a novel, compact, all-fiber laser system for advanced imaging techniques.
  • To demonstrate its capability for multiphoton imaging at significantly reduced excitation powers.
  • To enhance experimental flexibility and integration in various microscopy setups.

Main Methods:

  • Development of a gain-managed nonlinear (GMN) amplified Yb:fiber oscillator.
  • Integration of a pulse-picker for adjustable repetition rates (0.3-15 MHz).
  • Characterization of ultrashort pulse generation (39 fs) and peak power (0.83 MW).

Main Results:

  • The laser system delivers ultrashort pulses with high peak power.
  • Enables multiphoton imaging at excitation powers as low as 66 µW.
  • Demonstrates compatibility with two-photon, three-photon, and second harmonic generation microscopy.

Conclusions:

  • The compact all-fiber laser system offers a versatile and efficient solution for advanced microscopy.
  • Low excitation power requirements make it suitable for sensitive biological samples.
  • The system's design simplifies integration and expands applications in biophotonics.