Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Brain network construction and analysis for epilepsy: A methodology review.

Neural networks : the official journal of the International Neural Network Society·2026
Same author

ThermalGaussian++: Improving Alignment and Resolution for ThermalGaussian.

IEEE transactions on pattern analysis and machine intelligence·2026
Same author

Antiplatelet therapy in acute coronary syndrome: a systematic critical appraisal of current guidelines.

BMC cardiovascular disorders·2026
Same author

Anatomy-Aware MR-Imaging-Only Radiotherapy.

IEEE transactions on image processing : a publication of the IEEE Signal Processing Society·2026
Same author

An external validation of the population pharmacokinetic models of polymyxin B in critically ill patients.

European journal of clinical pharmacology·2025
Same author

A 3.55-µm Ultrathin, Skin-Like Mechanoresponsive, Compliant, and Seamless Ionic Conductive Electrode for Epidermal Electrophysiological Signal Acquisition and Human-Machine Interaction.

Exploration (Beijing, China)·2025

Related Experiment Video

Updated: May 9, 2025

Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development
13:01

Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development

Published on: April 10, 2016

33.8K

Zebrafish fluorescence imaging platform based on Bessel light sheet illumination.

Chuhui Wang1,2, Dongmei Su3, Ziheng Zhang2

  • 1Precision Medicine and Public Health, Tsinghua-Berkeley Shenzhen Institute, Tsinghua University, Shenzhen, Guangdong 518055, China.

Biomedical Optics Express
|May 5, 2025
PubMed
Summary

We created a 3D zebrafish imaging platform using Bessel light sheet fluorescence microscopy (LSFM). This system uses an electrically tunable lens (ETL) for auto-refocusing, improving image quality for studying live zebrafish structures and blood flow.

More Related Videos

Author Spotlight: High-Resolution 4D Light-Sheet Imaging and Virtual Reality in Zebrafish for Single-Cell Analysis of Heart Function
07:07

Author Spotlight: High-Resolution 4D Light-Sheet Imaging and Virtual Reality in Zebrafish for Single-Cell Analysis of Heart Function

Published on: January 5, 2024

1.1K
Visualizing Ocular Morphogenesis by Lightsheet Microscopy Using rx3:GFP Transgenic Zebrafish
07:40

Visualizing Ocular Morphogenesis by Lightsheet Microscopy Using rx3:GFP Transgenic Zebrafish

Published on: April 5, 2021

2.7K

Related Experiment Videos

Last Updated: May 9, 2025

Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development
13:01

Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development

Published on: April 10, 2016

33.8K
Author Spotlight: High-Resolution 4D Light-Sheet Imaging and Virtual Reality in Zebrafish for Single-Cell Analysis of Heart Function
07:07

Author Spotlight: High-Resolution 4D Light-Sheet Imaging and Virtual Reality in Zebrafish for Single-Cell Analysis of Heart Function

Published on: January 5, 2024

1.1K
Visualizing Ocular Morphogenesis by Lightsheet Microscopy Using rx3:GFP Transgenic Zebrafish
07:40

Visualizing Ocular Morphogenesis by Lightsheet Microscopy Using rx3:GFP Transgenic Zebrafish

Published on: April 5, 2021

2.7K

Area of Science:

  • Biomedical Imaging
  • Microscopy Technology
  • Zebrafish Model Systems

Background:

  • Three-dimensional (3D) imaging of biological samples like zebrafish is crucial for understanding complex structures and functions.
  • Traditional light sheet fluorescence microscopy (LSFM) faces challenges with defocusing during 3D acquisition due to optical path length changes.
  • Developing advanced imaging platforms is essential for high-resolution, in vivo visualization of biological processes.

Purpose of the Study:

  • To develop and validate a novel 3D zebrafish fluorescence imaging platform.
  • To address and overcome the defocusing issue in 3D LSFM using an electrically tunable lens (ETL).
  • To enhance image quality and enable precise visualization of zebrafish vasculature, lymphatic system, and blood flow.

Main Methods:

  • Utilized Bessel light sheet fluorescence microscopy (LSFM) for 3D imaging.
  • Integrated an electrically tunable lens (ETL) in the detection path for auto-refocusing.
  • Implemented a sample-moving axial scanning method and a novel auto-refocusing algorithm considering sample anisotropy.
  • Developed a magnification calibration method for precise volume synthesis and a hardware-based background elimination technique.

Main Results:

  • The ETL effectively corrected defocusing, providing satisfactory refocusing with low numerical aperture objectives.
  • The auto-refocusing method demonstrated robustness without mathematical signal limitations.
  • Magnification calibration improved the precision of 3D volume synthesis.
  • Hardware-based background subtraction significantly enhanced image quality, outperforming advanced denoising algorithms.
  • Successfully imaged live zebrafish lymphatic and vascular structures, and blood flow with high fidelity.

Conclusions:

  • The developed 3D LSFM platform with ETL-based auto-refocusing offers a reliable and high-performance solution for zebrafish imaging.
  • The system enables high-resolution, deconvolution-free visualization of intricate biological structures and dynamics in vivo.
  • This platform advancements contribute to improved understanding of zebrafish biology and disease models.