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Related Experiment Video

Updated: May 9, 2025

Profiling of Methyltransferases and Other S-adenosyl-L-homocysteine-binding Proteins by Capture Compound Mass Spectrometry CCMS

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Published on: December 20, 2010

A Benchtop Approach To Measuring S-AdenosylMethionine Metabolite Levels Using HILIC UPLC-MS.

Stuart Oehrle¹, Kathryn Sp Higginbotham², Erin D Strome²

¹Waters Field Lab, Chemistry Department, Northern Kentucky University, Highland Heights, KY 41099.

Journal of the Kentucky Academy of Science

|May 5, 2025

Summary

A new UPLC-MS method accurately measures S-adenosylmethionine (SAMe) in yeast. This technique enhances speed and sensitivity for studying SAMe production and its role in various health conditions.

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Last Updated: May 9, 2025

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Area of Science:

Biochemistry
Metabolomics
Analytical Chemistry

Background:

S-adenosylmethionine (SAMe) is a crucial methyl donor and enzyme substrate in cellular metabolism.
SAMe is widely used clinically and as a supplement for conditions like liver disease and osteoarthritis.
Industrial demand for SAMe, often produced in yeast (Saccharomyces cerevisiae), is increasing.

Purpose of the Study:

To develop a rapid, accurate, and sensitive method for measuring intracellular SAMe levels.
To support ongoing research into SAMe's cellular functions and optimize yeast production strains.

Main Methods:

A novel hydrophilic interaction liquid chromatography (HILIC) coupled with UltraPerformance liquid chromatography-mass spectrometry (UPLC-MS) benchtop method was established.
The method was applied to measure intracellular SAMe levels in Saccharomyces cerevisiae.

Main Results:

The UPLC-MS method provides high resolution, speed, and sensitivity for SAMe quantification.
This method offers a cost-effective and environmentally friendly alternative to existing techniques.

Conclusions:

The developed UPLC-MS method is suitable for measuring intracellular SAMe in yeast.
This analytical tool can advance research on SAMe metabolism and facilitate the development of yeast strains with enhanced SAMe production.
The method has potential for adaptation to measure other cellular metabolites.