Related Experiment Video

Updated: May 9, 2025

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development
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Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development

Published on: June 15, 2017

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CCL20 Expression via AKT-ERK1/2-AP1 Pathway in Mycoplasma Pneumoniae Infection: Implications for EMT and Cell

Xue Yang1,2, Daoyong Liao1, Ying Huang1

  • 1The Affiliated Nanhua Hospital, Department of Clinical Laboratory, Hengyang Medical School, University of South China, Hengyang, People's Republic of China.

Journal of Inflammation Research
|May 5, 2025
PubMed
Abstract

Insights

Mycoplasma pneumoniae infection boosts CCL20 production via the AKT-ERK1/2-AP1 pathway. This enhances cell migration and epithelial-mesenchymal transition (EMT), offering therapeutic targets for lung diseases.

Area of Science:

  • Immunology
  • Cell Biology
  • Respiratory Medicine

Background:

  • Mycoplasma pneumoniae is a key respiratory pathogen causing pneumonia and asthma.
  • Persistent infections can lead to lung fibrosis and airway abnormalities.

Purpose of the Study:

  • Investigate signaling pathways regulating CCL20 expression after M. pneumoniae infection.
  • Determine the impact of M. pneumoniae on cell migration and epithelial-mesenchymal transition (EMT).

Main Methods:

  • THP-1 cells were infected with M. pneumoniae to measure CCL20 expression.
  • Co-culture experiments with M. pneumoniae-infected THP-1 cells and bronchial epithelial cells assessed EMT and migration.

Main Results:

  • M. pneumoniae infection upregulated CCL20 in THP-1 cells via the AKT-ERK1/2-AP1 pathway in a time- and dose-dependent manner.
  • Co-culturing infected THP-1 cells with 16HBE cells promoted EMT and increased cell migration, linked to CCL20.

Conclusions:

  • CCL20 plays a role in M. pneumoniae-induced cell migration and EMT.
  • Understanding these mechanisms highlights potential therapeutic targets for M. pneumoniae-related lung diseases.
Keywords:
AKT-ERK1/2-AP1CCL20EMTM. pneumoniaecell migration

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