Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

UV–Vis Spectrometers

UV–Vis Spectrometers

The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...

Total Internal Reflection Fluorescence Microscopy

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.

Photoluminescence: Applications

Photoluminescence: Applications

Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...

Two-Dimensional Microscopy in Microbiology

Two-Dimensional Microscopy in Microbiology

Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...

Microbial Growth Measurement: Indirect Methods

Microbial Growth Measurement: Indirect Methods

Estimating microbial growth is essential for understanding population dynamics and environmental adaptations. Indirect methods provide valuable insights by measuring parameters such as turbidity, metabolic activity, and biomass, enabling efficient and reproducible assessments.During exponential growth, microbial cells scatter light proportionally to their biomass, a principle used in turbidity measurements. About one million cells per milliliter produce detectable scattering, which a...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Nanoscale Mechanisms of Piezoelectric Enhancement in MXene-Fluoropolymer Composites.

Nano letters·2026

Same author

Conductometric sensor for potassium ion profiling using lipophilic salt-incorporated non-toxic ion-selective membrane.

Journal of materials research·2025

Same author

Electrospinning of Well-Aligned P(VDF-TrFE) Fibers Using a Benign Solvent.

Macromolecular rapid communications·2025

Same author

The thickness-dependent response of aerosol-jet-printed ultrathin high-aspect-ratio electrochemical microactuators.

Soft matter·2024

Same author

3D-printed photothermal-responsive shape-memory polymer for soft robotic applications.

Chemical communications (Cambridge, England)·2024

Same author

Tailoring of Self-Healable Polydimethylsiloxane Films for Mechanical Energy Harvesting.

ACS applied energy materials·2024

Search research articles

Related Experiment Video

Updated: Jun 18, 2026

Thermal Measurement Techniques in Analytical Microfluidic Devices

Thermal Measurement Techniques in Analytical Microfluidic Devices

Published on: June 3, 2015

Temperature-dependent microfluidic impedance spectroscopy for non-invasive biofluid characterization.

Tom Wade¹, Sohini Kar-Narayan¹

¹Department of Materials Science and Metallurgy, University of Cambridge, 27 Charles Babbage Road, Cambridge CB3 0FS, United Kingdom.

Biomicrofluidics

|May 5, 2025

Summary

Temperature affects microfluidic impedance spectroscopy for remote health monitoring. Calibration is crucial for accurate sweat analysis, enabling detection of key biomarkers like chloride and lactate for personalized healthcare.

More Related Videos

Preparation and Testing of Impedance-based Fluidic Biochips with RTgill-W1 Cells for Rapid Evaluation of Drinking Water Samples for Toxicity

Preparation and Testing of Impedance-based Fluidic Biochips with RTgill-W1 Cells for Rapid Evaluation of Drinking Water Samples for Toxicity

Published on: March 7, 2016

Soft Lithographic Procedure for Producing Plastic Microfluidic Devices with View-ports Transparent to Visible and Infrared Light

Soft Lithographic Procedure for Producing Plastic Microfluidic Devices with View-ports Transparent to Visible and Infrared Light

Published on: August 17, 2017

Related Experiment Videos

Last Updated: Jun 18, 2026

Thermal Measurement Techniques in Analytical Microfluidic Devices

Thermal Measurement Techniques in Analytical Microfluidic Devices

Published on: June 3, 2015

Preparation and Testing of Impedance-based Fluidic Biochips with RTgill-W1 Cells for Rapid Evaluation of Drinking Water Samples for Toxicity

Preparation and Testing of Impedance-based Fluidic Biochips with RTgill-W1 Cells for Rapid Evaluation of Drinking Water Samples for Toxicity

Published on: March 7, 2016

Soft Lithographic Procedure for Producing Plastic Microfluidic Devices with View-ports Transparent to Visible and Infrared Light

Soft Lithographic Procedure for Producing Plastic Microfluidic Devices with View-ports Transparent to Visible and Infrared Light

Published on: August 17, 2017

Area of Science:

Biomedical Engineering
Analytical Chemistry
Wearable Technology

Background:

Remote health monitoring offers personalized and preventative care.
Sweat analysis via non-invasive sampling provides metabolic insights.
Accurate, rapid, and low-cost biofluid characterization is essential for widespread adoption.

Purpose of the Study:

Investigate temperature effects on microfluidic impedance spectroscopy (MIS) for biofluid analysis.
Assess MIS for simultaneous detection of both cationic and anionic species in sweat.
Validate MIS for accurate ionic concentration determination in the presence of neutral species.

Main Methods:

Utilized microfluidic impedance spectroscopy with a novel device architecture and analysis technique.
Examined the impact of varying temperatures on MIS measurements of ionic species.
Quantified concentrations of cationic (e.g., electrolytes) and anionic (e.g., chloride, lactate) species.

Main Results:

Microfluidic impedance spectroscopy measurements and concentration determinations are temperature-dependent.
Appropriate calibration is necessary for remote health monitoring devices subjected to temperature fluctuations.
The method successfully detected anionic species (chloride, lactate) alongside cationic species.
The presence of neutral species did not interfere with accurate ionic concentration measurements.

Conclusions:

Microfluidic impedance spectroscopy is a viable technique for non-invasive biofluid characterization.
Temperature compensation is critical for reliable remote health monitoring using MIS.
This technology supports the detection of important biomarkers for various health conditions.