Antiseptic activity of different ophthalmic formulations: an in vitro study

OBJECTIVE: The aim of the study was to evaluate the in vitro antiseptic power of nine ophthalmic preparations containing active ingredients with known antimicrobial properties: Polyhexamethylene biguanide 0.0003%; Hexamidine diisethionate 0.1%; chlorhexidine digluconate 0.02%; Povidone-iodine 0.6%; Hexamidine diisethionate 0.05% and polyhexanide hydrochloride 0.0001%; Hypochlorous acid 0.011%; Biosecur 2 g/100 mL; Self-preserved ozonated vegetable oil; Polyquaternium-133 0.1%. MATERIALS AND METHODS: Organisms were selected based on the current review of endophthalmitis literature: Staphylococcus aureus (ATCC 29213), Staphylococcus epidermidis (ATCC 35984), Pseudomonas aeruginosa (ATCC 15692) and Candida albicans (SC5314). The effect of the eye drops on bacterial cultures and yeast cells was evaluated using the Minimum Inhibitory Concentration assay and growth kinetics analysis. RESULTS: All tested ophthalmic solutions demonstrated antiseptic activity by reducing the microbial load of the selected organisms in vitro. At higher dilutions, some formulations maintained antiseptic properties. CONCLUSIONS: The antiseptic power of ophthalmic preparations tested in vitro is effective against species of microorganisms responsible for eye infections. Polyquaternium-133 0.1% has shown the best antiseptic activity, proving to inhibit or slow down the growth of microorganisms even at low concentrations.

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