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Related Concept Videos

Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

240
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
240
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

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The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
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Applications of IR Spectroscopy: Overview01:11

Applications of IR Spectroscopy: Overview

389
The non-destructive nature and ability to provide valuable chemical information make IR spectroscopy a versatile technique with broad applications in various scientific and industrial fields. IR spectroscopy is commonly used to identify and characterize organic and inorganic compounds. It provides information about the functional groups present in a molecule and the bonding between atoms. This helps in the structural elucidation of compounds during organic synthesis, pharmaceutical research,...
389
Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview01:13

Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview

234
Attenuated total reflectance (ATR) infrared spectroscopy is a powerful analytical technique used to study the composition of materials. It is widely employed in chemistry, materials science, forensic science, and other fields where sample characterization is required. ATR has several advantages over traditional transmission IR spectroscopy, including the requirement of little to no sample preparation and the ability to analyze a wide range of samples.
The ATR process begins by directing a beam...
234

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Assessing Stone Composition in Irrigation Fluid Using Raman Spectroscopy: A Blinded Comparative Study.

Orit Raz1, Iddo Pinkas2, Amir Cooper1

  • 1Department of Urology, Samson Assuta Ashdod University Hospital, Ashdod, Israel.

Journal of Endourology
|May 5, 2025
PubMed
Summary
This summary is machine-generated.

Raman spectroscopy (RS) can analyze kidney stone composition from microscopic fragments in irrigation fluid during ureteroscopy (URS). This method shows high concordance with traditional Fourier-transform infrared spectroscopy (FTIR), offering a potential new approach for stone analysis.

Keywords:
FTIRRaman spectroscopystone compositionurolithiasis

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Area of Science:

  • Urology
  • Spectroscopy
  • Materials Science

Background:

  • Kidney stone analysis is crucial for treatment and prevention.
  • Traditional methods require stone fragment retrieval, which can be challenging.
  • Minimally invasive techniques for stone analysis are desirable.

Purpose of the Study:

  • To evaluate the efficacy of Raman spectroscopy (RS) in determining kidney stone composition.
  • To analyze microscopic stone fragments present in irrigation fluid during ureteroscopy (URS) and laser lithotripsy.
  • To compare RS results with standard Fourier-transform infrared spectroscopy (FTIR) analysis.

Main Methods:

  • A prospective, blinded study was conducted on 22 patients undergoing URS with laser lithotripsy.
  • Irrigation fluid was collected, centrifuged, and analyzed using RS.
  • Stone fragments were simultaneously analyzed by FTIR in a separate laboratory.

Main Results:

  • Raman spectroscopy accurately identified the major stone component in 82.6% of cases.
  • High concordance was observed for calcium oxalate, calcium phosphate, and uric acid stones.
  • Discrepancies were noted in 17.4% of cases, with FTIR clarifying stone composition.

Conclusions:

  • Raman spectroscopy is a promising tool for analyzing kidney stone composition using irrigation fluid microparticles.
  • This technique shows strong agreement with FTIR, potentially eliminating the need for fragment retrieval.
  • Further research is needed to optimize this method for clinical use.