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Inflammatory Bowel Disease III: Diagnostic Studies and Management I-Nutritional Therapy01:30

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Various diagnostic tests are employed in the diagnostic process for Inflammatory Bowel Disease (IBD), particularly to differentiate between Crohn's disease and ulcerative colitis.
Diagnostic studies
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Microbial Biosensor for Sensing and Treatment of Intestinal Inflammation.

Duolong Zhu1, Jeffrey Galley1, Jason Pizzini1

  • 1Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, 77030, USA.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|May 5, 2025
PubMed
Summary

Scientists developed a novel biosensor to detect intestinal inflammation by targeting calprotectin. This biosensor not only diagnoses disease but also treats it by producing an anti-inflammatory cytokine, offering a dual-action approach for inflammatory bowel diseases.

Keywords:
IL10Zurbiosensorscalprotectinintestinal inflammation

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Area of Science:

  • Synthetic biology
  • Biosensor development
  • Gastroenterology

Background:

  • Intestinal inflammation detection often lacks targeted biomarkers.
  • Calprotectin is a clinically validated biomarker for intestinal inflammation.
  • Current biosensors lack combined diagnostic and therapeutic functions.

Purpose of the Study:

  • To develop an optimized biosensor targeting calprotectin for intestinal inflammation.
  • To create a biosensor with both diagnostic and therapeutic capabilities.
  • To utilize synthetic biology for in vivo disease detection and treatment.

Main Methods:

  • Engineered a biosensor using a zinc uptake regulator (Zur) controlled promoter.
  • Incorporated a memory circuit for detecting and recording inflammation in vivo.
  • Coupled biosensor activation with the production of Interleukin-10 (IL-10).

Main Results:

  • Biosensor activation levels correlated strongly with calprotectin levels in mouse colitis models.
  • The biosensor successfully detected and recorded intestinal inflammation in vivo.
  • Coupling with IL-10 production led to the resolution of chemically induced colitis.

Conclusions:

  • The developed biosensor effectively detects and responds to intestinal inflammation.
  • This synthetic biology approach offers a dual diagnostic and therapeutic strategy.
  • Utilizing clinically validated biomarkers like calprotectin is key for treating intestinal diseases.