Development of a Murine Model for Femoral Artery Anastomotic Stenosis

In vascular surgery, vascular anastomosis is a common reconstructive technique used to restore blood flow. However, anastomotic restenosis is a frequent postoperative complication, primarily caused by surgery-induced vascular injury, intimal hyperplasia, and inflammatory responses. The mouse femoral artery anastomosis model is widely used to investigate the mechanisms of anastomotic restenosis and vascular repair. Microscopically guided end-to-end femoral artery anastomosis allows precise simulation of vascular injury and repair processes following surgery, providing a reliable experimental tool for studying the pathological mechanisms related to restenosis. This study focuses on refining the surgical technique for femoral artery anastomosis in mice. Through refinements in surgical techniques and optimization of technical details, we have achieved a marked increase in the success rate and reproducibility of the model. Specific improvements include refined vascular handling techniques during surgery, the selection of suture materials, and the optimization of suturing methods to minimize anastomotic leakage and postoperative occlusion. The study also emphasizes the observation of intimal hyperplasia, vascular remodeling at the anastomotic site, and long-term vessel patency. Through this research, we provide a concise and efficient operational guide for performing mouse femoral artery anastomosis, offering reliable technical support for experimental studies in vascular surgery. This work lays a solid foundation for subsequent investigations into related mechanisms and evaluations of therapeutic intervention.