Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

6.2K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Temporal proteomic characterization of SARS-CoV-2 infected mouse lungs.

Molecular & cellular proteomics : MCP·2026
Same author

Bridging Ancestry-Stratified Bias in Pharmacogenomics AI: Toward Metabolomics-Inclusive Multi-Omics Precision Medicine.

Journal of personalized medicine·2026
Same author

Labile iron starvation in embryonic Kupffer cells aggravates MASH via mitochondrial failure and macrophage dysfunction.

Cell death & disease·2026
Same author

Performance validation of the KeraSkin™ reconstructed human epidermis (RhE) model according to OECD document no. 219 and exploratory optimization of its predictive capacity for in vitro skin corrosion testing.

Toxicology in vitro : an international journal published in association with BIBRA·2026
Same author

Trust-gated synthetic EEG augmentation reduces performance drops when generalizing to new patients.

NPJ digital medicine·2026
Same author

Exposure to trietazine causes cardiovascular and liver damage and impairs neurodevelopment through oxidative stress and inflammatory mechanisms in zebrafish and transgenic lines.

Comparative biochemistry and physiology. Toxicology & pharmacology : CBP·2026

Related Experiment Video

Updated: May 9, 2025

A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions
08:59

A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions

Published on: April 18, 2025

219

A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions.

Dongyoon Shin1, Dayeon Lee2, Young June Jeon2

  • 1Department of Medical Science, School of Medicine, CHA University; Department of Proteomic Research, CHA Future Medicine Research Institute.

Journal of Visualized Experiments : Jove
|May 5, 2025
PubMed
Summary

This study presents an integrated workflow for analyzing formalin-fixed, paraffin-embedded (FFPE) tissues using mass spectrometry (MS)-based proteomics. The method enables molecular discoveries in pancreatic cystic neoplasms from archived FFPE samples.

More Related Videos

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples
14:51

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples

Published on: November 13, 2021

5.1K
TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis
07:44

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis

Published on: June 8, 2020

12.5K

Related Experiment Videos

Last Updated: May 9, 2025

A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions
08:59

A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions

Published on: April 18, 2025

219
Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples
14:51

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples

Published on: November 13, 2021

5.1K
TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis
07:44

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis

Published on: June 8, 2020

12.5K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Pathology

Background:

  • Mass spectrometry (MS)-based proteomics offers comprehensive analysis of biological samples.
  • Formalin-fixed, paraffin-embedded (FFPE) tissues are valuable archival resources for proteomic studies.
  • Isolating regions of interest (ROIs) from FFPE tissues is crucial but lacks a streamlined proteomic workflow.

Purpose of the Study:

  • To present an integrated workflow for proteomic analysis of FFPE tissues.
  • To enable macrodissection of ROIs, sample preparation, and high-throughput MS analysis.
  • To demonstrate the applicability of the workflow for identifying molecular differences in pancreatic cystic neoplasms.

Main Methods:

  • Macrodissection of ROIs from FFPE tissue sections.
  • Suspension trapping-based proteomic sample preparation.
  • High-throughput mass spectrometry (MS) analysis.

Main Results:

  • High proteome coverage was achieved from macrodissected FFPE tissue ROIs.
  • Molecular differences between benign serous cystic neoplasms (SCN) and intraductal papillary mucinous neoplasms (IPMN) were identified.
  • The workflow successfully analyzed FFPE tissues from patients.

Conclusions:

  • The integrated workflow streamlines proteomic analysis of FFPE tissues.
  • This approach facilitates molecular discoveries in archived FFPE samples.
  • The method is applicable for advancing proteomic research in various FFPE-based studies.