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Related Experiment Video

Updated: May 12, 2025

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Acoustofluidic bioassembly induced morphogenesis for therapeutic tissue fabrication.

Byungjun Kang1, Eunseon Jeong2, Seung Yeop Han2,3

  • 1School of Mechanical Engineering, Yonsei University, Seoul, 03722, Republic of Korea.

Nature Communications
|May 5, 2025
PubMed
Summary

A novel acoustofluidic bioassembly technique precisely arranges cells, enabling the creation of advanced in vitro tissues with enhanced function and therapeutic potential for biomedical applications.

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Area of Science:

  • Biotechnology and Biomedical Engineering
  • Tissue Engineering
  • Cellular Mechanics

Background:

  • Replicating in vivo cell spatial distribution is crucial for in vitro tissue fabrication for therapeutic use.
  • Simultaneously controlling cell geometric alignment and aggregation during tissue fabrication presents significant technical challenges.

Purpose of the Study:

  • To introduce acoustofluidic bioassembly induced morphogenesis as a method for precise cell arrangement and tissue fabrication.
  • To demonstrate the capability of this technique in creating tissues with controlled nano-, micro-, and macro-structures.
  • To evaluate the functional and therapeutic enhancements of fabricated neuromuscular tissues.

Main Methods:

  • Utilized acoustofluidic cues to generate mechanical forces for precise cell arrangement.
  • Combined cell arrangement with subsequent in vitro and in vivo cultures to induce morphogenesis.
  • Fabricated neuromuscular tissues and assessed their structural, functional, and therapeutic properties.

Main Results:

  • Successfully created tissues with regulated nano-, micro-, and macro-structures using acoustofluidic bioassembly.
  • Fabricated neuromuscular tissues demonstrated enhanced contraction dynamics, electrophysiology, and therapeutic efficacy.
  • Demonstrated in situ application by fabricating artificial tissues directly at defect sites in living tissues.

Conclusions:

  • Acoustofluidic bioassembly induced morphogenesis offers a pioneering platform for advanced tissue fabrication.
  • This technique overcomes challenges in controlling cell spatial distribution for therapeutic tissue engineering.
  • The method shows significant potential for creating functional tissues for diverse biomedical applications.