Acoustofluidic bioassembly induced morphogenesis for therapeutic tissue fabrication.

Area of Science:

• Biotechnology and Biomedical Engineering
• Tissue Engineering
• Cellular Mechanics

Background:

• Replicating in vivo cell spatial distribution is crucial for in vitro tissue fabrication for therapeutic use.
• Simultaneously controlling cell geometric alignment and aggregation during tissue fabrication presents significant technical challenges.

Purpose of the Study:

• To introduce acoustofluidic bioassembly induced morphogenesis as a method for precise cell arrangement and tissue fabrication.
• To demonstrate the capability of this technique in creating tissues with controlled nano-, micro-, and macro-structures.
• To evaluate the functional and therapeutic enhancements of fabricated neuromuscular tissues.

Main Methods:

• Utilized acoustofluidic cues to generate mechanical forces for precise cell arrangement.
• Combined cell arrangement with subsequent in vitro and in vivo cultures to induce morphogenesis.
• Fabricated neuromuscular tissues and assessed their structural, functional, and therapeutic properties.

Main Results:

• Successfully created tissues with regulated nano-, micro-, and macro-structures using acoustofluidic bioassembly.
• Fabricated neuromuscular tissues demonstrated enhanced contraction dynamics, electrophysiology, and therapeutic efficacy.
• Demonstrated in situ application by fabricating artificial tissues directly at defect sites in living tissues.

Conclusions:

• Acoustofluidic bioassembly induced morphogenesis offers a pioneering platform for advanced tissue fabrication.
• This technique overcomes challenges in controlling cell spatial distribution for therapeutic tissue engineering.
• The method shows significant potential for creating functional tissues for diverse biomedical applications.