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Time-Course Transcriptome Analysis Unveils the CoFKF1-CoMYB4-CoFT1 Regulatory Module in Flowering Control of Camellia
Jindong Yan1,2,3,4,5, Qian Liu1,2,3,4,5, Purui Guo1,2,3,4,5
1State Key Laboratory of Utilization of Woody Oil Resource, Central South University of Forestry and Technology, Changsha, China.
Plant, Cell & Environment
|May 6, 2025
Summary
Camellia oleifera flowering is regulated by CoFKF1, which promotes early flowering by degrading CoMYB4. This enhances CoFT1 activity, providing insights into optimizing tea oil production.
Area of Science:
- Plant Biology
- Molecular Genetics
- Agricultural Science
Background:
- Camellia oleifera is a vital woody oil crop in Southern China.
- Understanding its flowering mechanisms is crucial for tea oil production.
- Current knowledge on C. oleifera flowering regulation is limited.
Purpose of the Study:
- To identify key regulatory genes controlling flowering time in C. oleifera.
- To elucidate the molecular pathway governing flowering induction.
- To provide a basis for optimizing flowering periods in C. oleifera.
Main Methods:
- Time-course transcriptome analysis to identify candidate genes.
- Ectopic expression of candidate genes to assess flowering time effects.
- Co-immunoprecipitation and ubiquitination assays to study protein interactions.
- Promoter binding assays to determine gene regulation.
Main Results:
- CoFKF1-like1 (CoFKF1) was identified as a central flowering regulatory gene.
- Ectopic expression of CoFKF1 induced early flowering in C. oleifera.
- CoFKF1 interacts with CoMYB4 in a blue-light-dependent manner, promoting CoMYB4 degradation.
- CoMYB4 directly represses the promoter activity of CoFT1.
Conclusions:
- CoFKF1 promotes flowering by destabilizing CoMYB4, which in turn enhances CoFT1 promoter activity.
- The CoFKF1-CoMYB4-CoFT1 module is a key pathway regulating flowering in C. oleifera.
- This study offers potential strategies for optimizing C. oleifera flowering periods.
