Protection of naringenin chalcone by a pathogenesis-related 10 protein promotes flavonoid biosynthesis in Marchantia polymorpha
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View abstract on PubMed
Summary
This summary is machine-generated.Pathogenesis-related 10 (PR10) proteins in liverworts promote flavonoid biosynthesis by stabilizing key intermediates. This study reveals PR10
Area Of Science
- Plant Biology
- Biochemistry
- Molecular Biology
Background
- Pathogenesis-related (PR) proteins are induced by stress and pathogens.
- Some PR proteins are linked to specialized metabolism, including proposed roles in anthocyanin biosynthesis.
- Limited data exist on PR protein functions in non-angiosperm species.
Purpose Of The Study
- To investigate the role of pathogenesis-related 10 (PR10) in flavonoid biosynthesis in the liverwort Marchantia polymorpha.
- To elucidate the mode of action of PR10 proteins in promoting flavonoid production.
- To determine if PR10 proteins contribute to flavonoid biosynthesis in non-angiosperm land plants.
Main Methods
- CRISPR/Cas9 gene editing to create Mppr10.5 mutants.
- RNA sequencing to analyze transcript abundance.
- Metabolite profiling to quantify flavonoid content.
- In vitro assays using recombinant MpPR10.5 protein to assess metabolite binding and stabilization.
Main Results
- Mppr10.5 mutants exhibited reduced levels of auronidin and flavones.
- MpPR10.5 protein demonstrated strong binding affinity for naringenin chalcone (NC), an unstable flavonoid intermediate.
- MpPR10.5 binding prevented spontaneous cyclization of NC in vitro.
- Downregulation of phenylpropanoid and flavonoid genes was observed in Mppr10.5 mutants.
Conclusions
- Pathogenesis-related 10 (PR10) proteins, exemplified by MpPR10.5, play a crucial role in promoting flavonoid biosynthesis.
- PR10 proteins likely function by binding and stabilizing unstable flavonoid intermediates like naringenin chalcone.
- This stabilization mechanism protects intermediates from degradation or unwanted reactions, facilitating downstream pathway progression.
- The findings suggest a conserved role for PR10 proteins in flavonoid metabolism across diverse land plant lineages.
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