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Updated: Jun 28, 2026

Direct Protein Delivery to Mammalian Cells Using Cell-permeable Cys2-His2 Zinc-finger Domains
Published on: March 25, 2015
Plasmid DNA delivery using arginine-rich cell-penetrating L/D-peptides containing α-aminoisobutyric acids
Tomohiro Umeno1, Hiroyasu Takemoto1, Makoto Oba1
1Graduate School of Medical Science, Kyoto Prefectural University of Medicine, 1-5 Shimogamohangi-cho, Sakyo-ku, Kyoto 606-0823, Japan. umeno@koto.kpu-m.ac.jp.
Abstract:
The relationship between intracellular uptake efficacy and the folding behavior of arginine-rich cell-penetrating L/D-peptides with α,α-disubstituted α-amino acids in plasmid DNA (pDNA) delivery was examined. Nano-sized complexes formed from pDNA and L/D-peptides efficiently traversed the cell membrane regardless of the peptide conformation. This finding represents a significant deviation from previously reported covalent cargo delivery methods using cell penetrating peptides with L- and D-amino acids.
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