Changes in cell motility proteins profile in HaCaT keratinocytes response to UVA exposure

A comparative analysis of HaCaT keratinocyte proteins has been performed after cell exposure to subtoxic doses (5 J/cm² and 25 J/cm²) of ultraviolet A (UVA) radiation. 930 proteins were identified by two or more unique peptides. More than half of all identified proteins (54.5%) demonstrated at least 2-fold increase in their relative content after HaCaT keratinocyte irradiation with a cumulative dose of 5 J/cm², while a decrease in the relative content was found only for 4 proteins. Irradiation of keratinocytes with a cumulative dose of 25 J/cm² resulted in a decrease in the proportion of up-regulated proteins (43.0%) and an increase in the number of down-regulated proteins (84). Among the proteins with increased relative content in HaCaT keratinocytes the most proteins were associated with "cell motility" (GO: 0048870), as well as regulation of cell shape and size, cell morphogenesis, and skin remodeling.