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Updated: May 8, 2025

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A Perylene-Based Bright Red-Emitting Probe for Wash-Free Cell Plasma Membrane Imaging Via Excimer Formation.

Mingshu Wu1, Yongling Zheng2, Ziwen Yan3

  • 1School of Chemical and Material Engineering, Hainan Vocational University of Science and Technology, Haikou, P. R. China. wumingshu@126.com.

Journal of Fluorescence
|May 6, 2025
PubMed
Summary

Researchers developed a novel perylene-based fluorophore (DAVP) for cell membrane imaging. This bright red-emitting agent forms excimers, enabling wash-free, high-contrast visualization of cell membranes with low cytotoxicity.

Keywords:
Cell MembraneExcimerFluorescent ProbePeryleneWash-free Imaging

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Area of Science:

  • Materials Science
  • Biotechnology
  • Chemical Biology

Background:

  • Excimer emission offers advantages for biological imaging due to red-shifted, broadened, and large Stokes-shifted characteristics.
  • Perylene-based fluorophores are explored for advanced imaging applications.

Purpose of the Study:

  • To develop a novel perylene-based amphiphilic fluorophore (DAVP) for effective and wash-free cell plasma membrane imaging.
  • To investigate the aggregation-induced emission properties of DAVP and its performance in surfactant micelles.

Main Methods:

  • Synthesis of a perylene-based amphiphilic fluorophore (DAVP).
  • Characterization of DAVP's fluorescence properties in aqueous solutions and surfactant micelles.
  • Evaluation of DAVP for cell plasma membrane imaging, assessing brightness, Stokes shift, solubility, cytotoxicity, and staining efficiency.

Main Results:

  • DAVP readily forms excimers, emitting intense red fluorescence with a large Stokes shift.
  • Fluorescence intensity of DAVP significantly increases upon molecular aggregation in aqueous solutions.
  • DAVP exhibits enhanced fluorescence in cationic and anionic surfactant micelles compared to aggregated states.
  • DAVP demonstrates bright red emission, high water solubility, low cytotoxicity, and wash-free capability for cell membrane imaging.

Conclusions:

  • DAVP is a promising perylene-based fluorophore for wash-free cell plasma membrane imaging.
  • The aggregation-induced emission and surfactant-enhanced fluorescence properties of DAVP enable high-contrast imaging.
  • DAVP offers a valuable tool for biological imaging with distinct advantages over existing agents.