Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Catalytically Perfect Enzymes

Catalytically Perfect Enzymes

The theory of catalytically perfect enzymes was first proposed by W.J. Albery and J. R. Knowles in 1976. These enzymes catalyze biochemical reactions at high-speed. Their catalytic efficiency values range from 108-109 M-1s-1. These enzymes are also called 'diffusion-controlled' as the only rate-limiting step in the catalysis is that of the substrate diffusion into the active site. Examples include triose phosphate isomerase, fumarase, and superoxide dismutase.

Most enzymes...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

An injectable Ce-MnCo LDH nanozyme gel with cascade catalytic activity for acute radiation proctitis in rats.

Biomaterials·2026

Same author

Towards the construction of a virtual yeast.

Nature·2026

Same author

Sturgeon roe protein hydrolysate alleviates cyclophosphamide-induced ovarian dysfunction in mice with reduced ovarian oxidative-inflammatory injury, granulosa cell apoptosis, and HPO axis-related endocrine imbalance.

Food & function·2026

Same author

Energy-translation coupling limits anaerobic yeast growth.

Nature communications·2026

Same author

Protein Structuromics Reveals a Loop-Controlled Half-Open Active Pocket Conformation Throughout Fe(II)/α-ketoglutarate-Dependent Dioxygenase Catalytic Cycle.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same author

Tunable particle-free region via acoustic force balance around an oscillating microbubble.

Ultrasonics·2026

Search research articles

Related Experiment Video

Updated: May 17, 2025

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology

Published on: December 15, 2017

Rational Design and Model Predictions for Optimized Elastase Production in Saccharomyces cerevisiae.

Xiufang Liu^1,2, Feiran Li³, Chufan Xiao^1,2

¹School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China.

ACS Synthetic Biology

|May 6, 2025

Summary

This summary is machine-generated.

Engineered Pseudomonas aeruginosa elastase in yeast using systems biology approaches. This research optimized protease production, showcasing Saccharomyces cerevisiae as a versatile host for industrial enzyme manufacturing.

Keywords:

S. cerevisiae elastase metabolic engineering protease production target prediction

More Related Videos

Optimization of the Epimedii Folium Mutton-Oil Processing Technology and Testing Its Effect on Zebrafish Embryonic Development

Optimization of the Epimedii Folium Mutton-Oil Processing Technology and Testing Its Effect on Zebrafish Embryonic Development

Published on: March 17, 2023

Quantifying the Modulation of Elastase Enzyme Activity Through Colorimetric Analysis

Quantifying the Modulation of Elastase Enzyme Activity Through Colorimetric Analysis

Published on: January 17, 2025

Related Experiment Videos

Last Updated: May 17, 2025

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology

Published on: December 15, 2017

Optimization of the Epimedii Folium Mutton-Oil Processing Technology and Testing Its Effect on Zebrafish Embryonic Development

Optimization of the Epimedii Folium Mutton-Oil Processing Technology and Testing Its Effect on Zebrafish Embryonic Development

Published on: March 17, 2023

Quantifying the Modulation of Elastase Enzyme Activity Through Colorimetric Analysis

Quantifying the Modulation of Elastase Enzyme Activity Through Colorimetric Analysis

Published on: January 17, 2025

Area of Science:

Biotechnology
Microbial Engineering
Enzyme Production

Background:

Pseudomonas aeruginosa elastase is a valuable metalloprotease with industrial applications.
Production challenges arise from its pathogenic origin and complex protein folding.
Saccharomyces cerevisiae (yeast) offers potential as a host for heterologous protein expression.

Purpose of the Study:

To engineer improved variants of Pseudomonas aeruginosa elastase in Saccharomyces cerevisiae.
To utilize systems biology and rational design for enhanced protease production.
To identify genetic targets for optimizing yeast cell factory performance.

Main Methods:

Rational design targeting nonfunctional elastase regions (propeptide, signal peptide cleavage, N-glycosylation).
Integration of the yeast protein secretory model (pcSecYeast) with production characteristics.
Identification and validation of 75 genetic targets through model prediction and production feature analysis.
Gene overexpression (POS5) and knockout (TES1, VPS10) strategies.

Main Results:

Development of several engineered elastase variants with improved properties.
Overexpression of POS5 resulted in a 2.43-fold increase in protease activity.
Knockout of TES1 or VPS10 genes further optimized elastase production.
Validation of 75 genetic targets for enhanced protease yield.

Conclusions:

Systems biology is effective for developing yeast cell factories for protease production.
Saccharomyces cerevisiae is a versatile host for biotechnological applications, including enzyme manufacturing.
Engineered elastase variants show significant potential for industrial use.