Related Experiment Video
Updated: May 20, 2026

Titration of Human Coronaviruses Using an Immunoperoxidase Assay
Published on: April 28, 2008
Protocol for detection of Oropouche viruses from human serum
Laura S Perez-Restrepo1, Cole Knuese2, Vanessa Vargas1
1Global Health Institute One Health Colombia, National University of Colombia, Medellin 050032, Colombia.
None:
Oropouche virus (OROV), an arthropod-borne virus, has reemerged in the Americas. In 2023-2024, the largest quantitative reverse-transcription PCR (RT-qPCR)-confirmed outbreak of OROV was reported and was associated with severe clinical outcomes for the first time. Moreover, a dramatic increase in cases is unfolding in early 2025. Here, we provide a protocol for detecting OROV from human serum. We describe steps for viral isolation, titration, and an in-house RT-qPCR validated in the field. We then detail procedures for measurement of anti-OROV-neutralizing antibodies. For complete details on the use and execution of this protocol, please refer to Ciuoderis et al.1 and Usuga et al.2.
Related Concept Videos
Viral Recombination
Investigation of Disease Outbreaks
Bacterial Gastroenteritis
Hepatitis

