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PBS-DLS: A Novel Ultrasensitive Dynamic Light Scattering Immunoassay
Hao Fang1, Tian Gong2,3,4, Yu Su1
1State Key Laboratory of Food Science and Resources, School of Food Science and Technology, Nanchang University, Nanchang 330047, P.R. China.
ACS Applied Materials & Interfaces
|May 6, 2025
Summary
A new universal polyvalent biotin-streptavidin cross-linking aggregation coupled with dynamic light scattering (PBS-DLS) offers ultrasensitive detection. This method achieves attomolar sensitivity in 15 minutes, ideal for point-of-care testing.
Area of Science:
- Biotechnology
- Analytical Chemistry
- Nanotechnology
Background:
- Current ultrasensitive detection methods often require complex equipment and lengthy procedures.
- There is a need for faster, simpler, and more sensitive diagnostic tools.
Purpose of the Study:
- To develop a novel, ultrasensitive, and rapid detection strategy for molecular recognition events.
- To demonstrate the potential of this new method for point-of-care (POC) diagnostics.
Main Methods:
- Utilized universal polyvalent biotin-streptavidin cross-linking aggregation (PBS) combined with dynamic light scattering (DLS).
- Developed a DLS nanoprobe with controllable size and quantity for tunable sensitivity.
- Applied the PBS-DLS method to detect SARS-CoV-2 nucleocapsid protein and malachite green.
Main Results:
- Achieved tunable sensitivity down to attomolar levels with a broad detection range.
- Reduced detection time to approximately 15 minutes.
- Successfully validated the PBS-DLS immunosensor in complex sample matrices, outperforming conventional methods.
Conclusions:
- The PBS-DLS strategy provides a simple, rapid, and ultrasensitive platform for molecular detection.
- This novel approach shows significant promise for developing advanced bioanalytical methods and POC assays.
