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Updated: May 9, 2025

Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement
Published on: July 26, 2017
Myotubularin related protein 7, a novel STIM1 binding protein
Ning Dai1, Jody Groenendyk1, Marek Michalak1
1Department of Biochemistry, University of Alberta, Edmonton, AB T6G 2H7, Canada.
None:
Stromal interaction molecule 1 (STIM1) is a Ca2+ sensor in the endoplasmic reticulum (ER) membrane. The protein plays a crucial role in store-operated Ca2+ entry (SOCE) by transducing ER Ca2+ depletion signals to Ca2+ release-activated Ca2+ channel protein 1 (ORAI1) at the plasma membrane. Myotubularin related protein 7 (MTMR7) is a lipid phosphatase that dephosphorylates phosphoinositides. Using yeast two-hybrid analysis, immunoprecipitation and fluorescence microscopy, we discovered that MTMR7 interacts with STIM1 at the ER. These observations identified MTMR7 as a novel STIM1-binding protein that bridges myotubularins and phosphoinositide signaling with SOCE. Our research revealed a novel link between Ca2+ signaling and phosphoinositide biology, positioning MTMR7 as a potential marker or drug target for SOCE related human pathophysiology.
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