Innovative applications of SERS in precision medicine: In situ and real-time live imaging

Surface-enhanced Raman scattering (SERS), a molecular spectroscopic technique with high sensitivity and specificity, has demonstrated groundbreaking potential in precision medicine in recent years. This review systematically summarizes recent advancements in SERS technology for in situ and real-time live imaging, focusing on its core value in early tumor diagnosis, intraoperative navigation, drug delivery monitoring, and dynamic pathological analysis. By optimizing nanoscale probe design-including targeted functionalization, enhanced biocompatibility, and integration with imaging systems-SERS overcomes the sensitivity and spatiotemporal resolution limitations of traditional imaging techniques, enabling precise capture and dynamic tracking of molecular events in live biological environments. The article further analyzes challenges in clinical translation, such as signal stability in complex biological environments, multimodal imaging coordination, and standardized data processing methods. Future directions for personalized therapy and intelligent integrated diagnostics are also discussed.