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Comparative study on the effects of saline and lactated ringer's solution as washing solutions for red blood cell
Xiaomin Su1, Xin Liu2, Guoquan Zhang1
1Shaanxi Province Blood Center, Institute of Xi'an Blood Bank, 407# Zhuque Ave, Xi'an, Shaanxi Province 710061, China.
Background And Objective:
The objective of this research was to analyze and compare the quality, proteomics, and metabolomics of washing red blood cells (WRBCs) with saline and lactated ringer's solution (LRS) as washing solutions during storage.
Methods:
The units were divided into three bag aliquots, with one group serving as the control and not undergoing washing, another group being washed with sodium LRS, and a third group being washed with sodium chloride solution. These aliquots were stored for a period of 5 weeks, with various assays assessing the RBC storage lesion at weeks 0, 1, 3, and 5. Subsequently, the RBCs were subjected to proteomic and nontargeted metabolomic analyses at the conclusion of the storage period.
Results:
After testing, we found that none of the samples exceeded national standards. The concentrations of Na+ , K+ , glucose (GLU), LDH, and 2,3-DPG in washed RBCs were lower compared to unwashed RBCs during storage. Na+ and GLU levels decreased while K+ and 2,3-DPG levels increased in each group. Both the LRS and saline groups showed a decrease in the accumulation of RBC storage lesions during storage. In addition, results obtained from this integrated proteomics and metabolomics study showed changes in some proteins and metabolites. These pathways may be the core pathways of changes in the two sample groups.
Conclusions:
This study indicate that the performance of RBCs washed with LRS surpasses that of those washed with Saline in certain aspects. They are safe and effective when used as washing solutions for blood transfusions.
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