Related Experiment Video
Updated: Jun 20, 2026

Three-Dimensional Culture of Murine Colonic Crypts to Study Intestinal Stem Cell Function Ex Vivo
Published on: October 11, 2022
Stemness in flux: Dissecting intestinal crypt organization with multimodal approaches
Maria Azkanaz1, Dimitrios Laskaris1, Jacco van Rheenen1
1Division of Molecular Pathology, Oncode Institute, the Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, the Netherlands.
Abstract:
In this issue of Developmental Cell, Banjac et al. integrate lineage tracing, single-cell RNA sequencing, and mathematical modeling to reveal that stem cells at the crypt base drive the decision between secretory and absorptive lineage commitment. Their findings highlight the central role of crypt-bottom Lgr5+ cells in maintaining intestinal epithelium homeostasis.
More Related Videos
06:31Author Spotlight: Studying the Epithelial Effects of Intestinal Inflammation In Vitro on Established Murine Colonoids
Published on: June 2, 2023
10:39Author Spotlight: The 3D Culturing of Organoids from Murine Intestinal Crypts and a Single Stem Cell for Organoid Research
Published on: April 7, 2023
Related Concept Videos
Renewal of Intestinal Stem Cells
Role Of Notch Signalling In Intestinal Stem Cell Renewal
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...